摘要
In order to propagate and cultivate Sequioa sempervirena,taking 63 clones introduced from France as samples,the study on techniques and measures of tissue culture of Sequioa sempervirens was carried out from the aspects of sample preparing,explant differentiation inducting,bud propagating,and root generating.The results showed that the optimum medium for inducing bud differentiation was MS+6-BA1.5 mg·L-1+KT2.0 mg·L-1,root medium was 1/2MS+IBA1.0 mg·L-1+NAA2.0 mg·L-1.
In order to propagate and cultivate Sequioa sempervirena,taking 63 clones introduced from France as samples,the study on techniques and measures of tissue culture of Sequioa sempervirens was carried out from the aspects of sample preparing,explant differentiation inducting,bud propagating,and root generating.The results showed that the optimum medium for inducing bud differentiation was MS+6-BA1.5 mg·L^(-1)+KT2.0 mg·L^(-1),root medium was 1/2MS+IBA1.0 mg·L^(-1)+NAA2.0 mg·L^(-1).
出处
《林业科学研究》
CSCD
北大核心
2005年第1期102-105,共4页
Forest Research
基金
国家林业局"948"项目"北美红杉优良种源及无性系引进"(项目编号99 4 05)
关键词
北美红杉
无性系
组织培养
Sequioa sempervirens
clome
tissue culture
