摘要
采用IHA和ELISA对纯化的日本血吸虫成虫31/32KD抗原与虫卵可溶性抗原的诊断特异性和敏感性进行比较。结果表明,前者的特异性高,检测正常人血清未见假阳性反应,与肝吸虫或肺吸虫病人血清无交叉反应;治后一年的阴转率达70％,故疗效考核价值较高。二种抗原的敏感性无显著差异。
A preliminary study on immunodiagnosis of schistosomiasis japonica by the homologous 31/32KD adult worm protein was carried out by using IHA and routine ELISA. Advantages of the purified 31/ 32KD adult worm antigen compared with the soluble egg antigen (SEA) were : 1) Sensitivity of 31/ 32KD antigen was as high as SEA. This antigen produced positive rates of 100% by both methods in acute patients,and 95% by IHA 91. 7% by routine ELISA in chronic patients. 2) No false or cross reaction was produced by 31/32KD antigen, while some nonspecific reactions were emerged with normal subjects, patients with paragonimiasis and patients with clonorchiasis by SEA. 3) One year after treatment, 77. 5% of the positves turned negative by 31/32KD antigen-based IHA while only 52. 5% of the positives turned negative by SEA-based IHA.
出处
《湖南医科大学学报》
CSCD
1990年第4期313-316,共4页
Bulletin of Hunan Medical University
基金
TDR/WHO资助课题
关键词
日本血吸虫
抗原
血吸虫病
诊断
schistosoma japonicum
antigens
31/32KD adult worm antigen
soluble egg antigen
immunodiagnosis
comparative study
