摘要
本文用ＳＤＳ－ＰＡＧＥ及免疫印迹法测定人肝癌相关抗原──ＨＬ＿２抗原分子量为５０ｋｄ。ＡＢＣ免疫组织化学染色１２７例肿瘤及肝病变组织石蜡切片显示ＨＬ＿２单抗与原发性肝细胞癌组织有较高的阳性反应率（６２．７％），与胃癌组织也有较多的交叉反应（８／１０），但与消化道其他癌和肝炎组织仅有少量的交叉反应，与肝硬化及其他系统的癌组织无交叉反应，说明单抗ＨＬ＿２对原发性肝细胞癌的诊断及与其它系统肿瘤的鉴别诊断有一定的临床应用价值．
Molecular weight of human hepatocel-lular carcinoma (HOC)-associated antigen-HL_2 antigen was detected to be about 50 000 dalton by SDS-PAGEand Western blot.One hundred and twenty-sevenparaffin sections of cancers and pathological liver tis-sue were tests by ABC immunohistochemical staining。MAb HL_2 reacted with 62.7%HCC and also with 8 ofthe 10 stomach carcinomas but only with a few otherdigestive system carcinomas(pancreas carcinoma , rec-tum carcinoma and duodenum carcinoma)and hepatitis. MAb HL_2 was negative in hepatocirrhosis andother tumors(vesica carcinoma,skin carcinma,breast carcinoma , neurogliocytoma and carcinoma ofthe lung).The results suggest that MAb HL_2 has val-ues in diagnosis of HCC and differential diagnosis be-tween HCC and other tumous。 HL_2 antigen might be anew tumor-associated antigen(TAA).Further studyof HL_2 antigen will significantly show the actions ofTAA in the occurrence and development of tumor.
出处
《华西医科大学学报》
CSCD
1994年第1期35-37,共3页
Journal of West China University of Medical Sciences
基金
四川省卫生厅资助
关键词
单克隆抗体
肝细胞癌
免疫组织化学
Monoclonal antibody HL_2 Hepotocellular carcinoma Immunohistochemical staining
