摘要
用新鲜人肝癌组织细胞悬液免疫,获得2株抗人肝癌细胞单克隆抗体(HAb-20,HAb-21),应用免疫组化方法对部分正常肝、肝硬变、原发性肝癌等组织进行定位。结果原发性肝癌阳性率分别为91.1％和63.1％,而在正常肝、肝硬化无相应抗原表达。2株抗体在胆管上皮、胃癌及结肠癌等组织存在不同程度的交叉,结果表明2株抗体对肝癌组织具有明显的相关性。
Monoclonal antibodies (HAb-20 and HAb-21) against human primary hepatocrllular carcinoma were prepared by fusion of splenic cells from BALB/C mice previously immunized with cell suspension of fresh surgically removed hepatoma tissues.Immunohistochemical localization was performed with ABC method in tissues of normal liver,cirrhetic liver,hepatocellular carcinoma and its surrounding tissues,etc.Positive HAb-20 stainiing was detected in 82/90 cases (91.1%) of hepatoma and HAb-21 was detected in 36/57 cases (63.1%).Positive hepatoma staining was either membranous or cytoplasmic.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
1989年第6期402-405,共4页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金
关键词
原发性肝癌
单克隆抗体
免疫组织化学
Primary hepatocellular carcinoma Monoclonal antibody Immunohistochemistry
