摘要
多数生物活性分子,如酶,抗体及药物在室温下接受辐射能后其生物活性和药力会受到不同程度的损伤。低温(-78℃)辐射对酶的辐射失活有不同程度的保护作用。为此,嘉悦憅等人曾发展了一种低温辐射包埋技术。另外,有一些酶(如过氧化氢酶、辣根过氧化物酶等)还具有辐射后失活效应,即受辐照酶样品取出辐射场后其活性仍随存放时间而不断下降。低温可以减缓后失活速率但难以完全排除。为避免低温操作的麻烦和辐射失活与后失活的损失,作者曾使用一种室温预辐射包埋技术。这一技术是将包埋过程分为两步:先使单体溶液在不断通氧下室温预辐照,然后在辐射场外与适当酶混合,通氮使其聚合,达到物理包埋的效果。
Most of bioactive species such as enzyme and antibody will be damaged and lose part of their bioactivity when they are irradiated by ionizing radiation at room temperature. Low temperature could somewhat control the effect, so kaetsu and his co-workers developed an entrapping technique at low temperature ( - 78℃). On the other hand, some enzymes such as catalase and house radish peroxidase have so-called radiation-induced post-deactivation effect as well. In order to avoid the trouble in performance and loss of bioactivity, a physical entrapping of bioactive species via preirradiation polymerization at room temperature were developed in the Authors' lab. In this work we developed this technique. The glucose oxidase was entrapped in polyacrylamide hydrogel induced by pre-irradiation. Experiments under several different conditions such as absorbed dose and concentrations of enzymes, monomer and crosslinking agent were carried out. The results showed that this technique can be used extensively in various enzyme.
出处
《高分子学报》
SCIE
CAS
CSCD
北大核心
1992年第6期732-735,共4页
Acta Polymerica Sinica
关键词
葡萄糖氧化酶
丙烯酰胺
辐射聚合
Glucose oxidase, Acrylamide, Radiation polymerization, Physical entrapping
