摘要
目的：为探讨肾综合征出血热病毒（ＨＦＲＳＶ）能否感染体外培养的人肾小球系膜细胞及在其中复制，能否引起细胞病变。方法：用ＨＦＲＳＶ７６－１１８株、ＳＲ－１１株和陈株分别攻击体外传代培养的人肾小球系膜细胞。结果：于培养第一代即在受染细胞的胞浆中检出特异性病毒抗原，阳性细胞数及胞浆内抗原的含量随传代次数的增加而增加．感染细胞在光镜下未见明显病理损害。结论：ＨＦＲＳＶ可以感染体外培养的人肾小球系膜细胞并可以在其中复制。
Objective : To study the replication of hemorrhagic fever with renal syndrome(HFRS)virus incultured human glomerular mesangial cells and the effects of HFRS virus on human glomerular mesangialcells. Methods :The 76-118 strain, SR-11 strain and Chen strain of HFRS virus were inoculated into the cul-tured human glomerular mesangial cells, Results : It was found that the virus antigens could be detected inthe first passage by immunofluorescence technique, and that the increments of the detectable positive cellsand viral antigens in the cytoplasm of infected cells were consistent with the prolongation of culture and fre-quence of passages. By phase contrast microscope, no obvious difference in morphology was found betweenthe infected cells and the control cells in the course of three times. Conclusions: These results indicated thatHFRS virus could infect cultured human glomerular mesangial cells and replicate in it。
出处
《第四军医大学学报》
1994年第6期447-449,共3页
Journal of the Fourth Military Medical University
关键词
流行性出血热
病毒
肾小球膜
肾病综合征
hemorrhagic fever, epidemic
virus replication
glomerular mesangium
