摘要
目的：为进一步研究运动训练过程中心肌线粒体功能的变化．方法：观察不同时间跑台运动对大鼠心肌线粒体Ｈ＋转运ＡＴＰ酶活性及膜流动性的影响．结果：大鼠在运动至１５ｍｉｎ时，心肌线粒体Ｈ＋转运ＡＴＰ酶活性及寡霉素的抑制率与对照组比较无明显变化，线粒体膜的荧光偏振度明显降低（０．２７９±０．００８ｖｓ０．２６７±０．００７，Ｐ＜０．０５）；大鼠在运动至衰竭时，心肌线粒体Ｈ＋转运ＡＴＰ酶活性及寡霉素的抑制率与对照组比较均明显降低［６．８６±１．６４ｖｓ４．５６±０．４６μｍｏｌ／（ｍｉｎ·ｍｇｐｒ）和（９４．２９±３．７４）％ｖｓ（４８．１９±１４．３３）％，Ｐ＜０．０１］，线粒体膜荧光偏振度降低显著（０．２７９±０．００８和０．２６７±０．００７ｖｓ０．２５８±０．０１０，Ｐ＜０．０１）．结论：一定强度下不同时间的运动训练，不足以使心肌线粒体功能发生明显改变，但一次力竭性运动可以导致心肌线粒体膜的流动性增强，Ｈ＋转运ＡＴＰ酶活性降低．
Objective:To study the changes of mitochondrial function in myocardiocytes during exercise. Methods:Twenty-seven male SD rats were randomized into 3 groups. Group 1 served as the control group,and rats of Group 2 and 3 ran on treadmill at a speed of 27 m/min and an angle of 8 for 15 min and for as long as physical exhaustion occurred,respectively. Immediately after exercise , hearts were collected for examina-tion of the fluidity of submitochondrial membrane in myocardiocytes,the activity of H+ -transporting ATPase and its sensitivity to oligomycin,Results:When rats ran up to 15 min,the fluidity of submitochondrial mem-brane of myocardiocytes was significantly greater than that of the control(0.279±0.008 vs 0.267±0.007,P<0.05),and there was no significant difference between Group 2 and the control in the activity of H+ -trans-porting ATPase in mitochondria of myocardiocytes and its sensitivity to oligomycin.When rats ran to physi-cal exhaustion,there was a significant reduction in the activity of H+ -transporting ATPase in mitochondria of myocardiocytes and its sensitivity to oligomycin[6.86± 1.64 vs 4.56±0.46μmol/(min·mg protein)and 94.29±3.74 vs 48.19±14.33%, P<0.01],and the increase in membrane fluidity was greater than that of Group 1 and Group 2(0.279±0.008 and 0.267±0.007 vs 0. 258±0.010,P<0.01),respectively. Conclu-sions:The aforementioned findings indicated that one bout of exhaustive exercise might cause changes in the fluidity of mitochondrial membrane in myocardiocytes and a significant reduction in the activity of H+ -trans-porting ATPase while physical training of some intensity was not adequate in inducing apparent changes in the function of mitochondria in myocardiocyte.
出处
《第四军医大学学报》
1994年第2期105-107,共3页
Journal of the Fourth Military Medical University
关键词
运动试验
线粒体
心脏
ATP酶
exercise test
mitochondria
heart
H+ -transporting ATPase
membrane fluidity
