摘要
本文观察了二十碳五烯酸(EPA)对大鼠脾脏天然杀伤(NK)细胞活性的影响,并对其可能的作用机理进行了探讨,结果表明,EPA乙酯(EPA-E)ig可明显降低脾脏NK细胞活性和白三烯B_4(LTB_4)的产生从脾细胞中除去粘附性细胞后,EPA-E对NK细胞活性的抑制作用消失,而这种非粘附脾细胞几乎没有产生LTB_4的能力,另外,外源性LTB_4可部分恢复EPA-E抑制的NK细胞活性。
The effect of eicosapentaenoic acid(EPA),a polyunsaturated fatty acid,on natural killer(NK)cell activity in rat whole spleen cells(WSC)and rat non-adherent spleen cells(NASC)was studied.Oral ingestion of EPA ethyl ester(EPA-E)depressed the WSC NK activity and the production of leukotriene B4(LTB4)in WSC significantly.The depressed WSC NK activity by ingested EPA-E was partly resumed by LTB4 added exogenously,and it was recovered significantly at 10 nmol·L-1 of LTB4.On the contrary,EPA-E ingestion showed no inhibitory effect on the NASC NK activity.Then the capacity ofLTB4 production was compared between WSC and NASC when stimulated with calcium ionophore A23187.WSC produced a significant amount of LTB4,while NASC produced only a negligible amount of it.The results indicate that the inhibitory effect of EPA ingestion on NK activity may be partly mediated by the inhibitory effect on production of LTB4 in rat spleen adherent cells.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
1993年第3期186-189,共4页
Chinese Journal of Pharmacology and Toxicology
关键词
二十碳五烯酸
天然
杀伤细胞
Eicosapentaenoic acid Natural killer cells Leukotriene B
