摘要
用3对水稻植株高度近等基因对,研究了株高基因时GA_3敏感性和酶的影响。结果表明,株高基因在苗期对GA_3敏感性强弱顺序为eui>sdl>Sdl>sds(t);其中粳稻的Sdl敏感性强于籼稻的。用胚乳培养法快速鉴别水稻株高基因对GA_3敏感性的结果与幼苗鉴定的一致,说明这种方法是有效的。在酶活性方面,4d的幼苗中高秆品系的过氧化物酶活力比矮秆品系的高,而8d的幼苗中矮秆的活力反而比高秆的强。经GA_3处理4d和8d后,对GA_3敏感的品系过氧化物酶活力较低。过氧化物酶同工酶带8和酯酶同工酶带7与株高基因对GA_3的敏感性有关。
Sensitivities of different height genes of rice to GA_3 and their relationships with enzymes were studied using three pairs of isogenic lines. The results indicated that the sensitivities of the genes to GA_3 were in the order: eui>sdl>Sdl>sds(t). The gene sdl was more sensitive in indica than in japonica. Sensitivities of the endosperms with the different height genes to GA_3 quitely accorded with these of the seedlings. Assay of peroxidase showed that activity of the enzyme was higher in the tall lines than in the short lines in seedlings of 4 days. and higher in the short lines than in the tall ones in seedlings of 8 days. The enzyine was lower activity in sensitive lines treated with GA_3 for 4 and 8 days. The isozyme band 8 of peroxidase and the band 7 of esterase on the electrophoresis gel were related to the sensitivity of the height genes to GA_3.
出处
《中国水稻科学》
CAS
CSCD
北大核心
1993年第3期143-147,共5页
Chinese Journal of Rice Science
基金
国家自然科学基金
关键词
水稻
株高基因
敏感性
酶
赤霉素
Rice
Plant height genes
Sensitivity to GA_3
Enzyme
