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利用等电聚焦电泳研究黄单胞菌分类 被引量:2

A STUDY ON CLASSIFICATION OF XANTHOMONAS BY ISOELECTRIC FOCUSING
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摘要 对黄单胞菌的4个种及29个致病变种的代表菌株进行了等电聚焦电泳研究,发现所测定的黄单胞菌种间、致病变种间蛋白质图谱差别很大。电泳结果经聚类分析表明,某些致病变种间的差别并不一定比种间的差别小,说明某些致病变种可以上升到种这一分类地位。引起细菌性黑颖病的黄单胞菌禾谷致病变种、小麦致病变种、大麦致病变种间差别很小,仅有6条蛋白质谱带的差别。因此这3个致病变种的分类地位需重新考虑,同时说明等电聚焦电泳对黄单胞菌种下分类具有一定指导意义。 Studing Xanthomonas strains of 4 species and 29 pathovars by isoelectric focusing,itwas shown that the protein patterns were very different among the species and pathovars.Through cluster analysis of IEF date,it was found that the protein pattern differences among cer-tain pathovars were no fewer than that among the species.It revealed that some pathovarscould become to be species.There were few differences of 6 protein bands among X.campes-tris pv.cerealis,X.campestris pv.undulosa and X.campestris pv.hordei which cause bac-terial black cheff,so the classification of the three pathovars need to be re-considered.It alsoshowed that isoelectric focusing could be used to classify Xanthomonas below species.
出处 《微生物学报》 CAS CSCD 北大核心 1993年第1期7-12,共6页 Acta Microbiologica Sinica
关键词 分类 等电聚焦 电泳 黄单胞菌属 Classification of Xanthomonas Isoelectric Focusing
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参考文献3

  • 1陶慰孙,蛋白质分子基础,1986年
  • 2何忠效,等电聚焦,1985年
  • 3程光胜,微生物学实验法,1983年

同被引文献14

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  • 9张鼎鼎,邹丽芳,赵梅勤,邹华松,陈功友.hrcQ基因决定水稻条斑病菌在非寄主烟草上的过敏性反应和在寄主水稻上的致病性[J].中国水稻科学,2011,25(1):11-18. 被引量:7
  • 10吴波,何明雄,冯红,张艳,胡启春,张义正.运动发酵单胞菌限制-修饰系统缺失突变株的构建及其性质分析(英文)[J].应用与环境生物学报,2013,19(2):189-197. 被引量:3

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