摘要
【目的】探讨端粒酶活性与肺癌临床病理特征的关系,以及端粒酶活性强度与外周血T淋巴细胞 嗜银蛋白(Ag NORs)含量的关系。【方法】采用PCR TRAP方法检测48例肺癌组织和28例癌旁组织端粒 酶活性,并用免疫图像分析的方法术前检测20例病人的外周血T淋巴细胞Ag NORs含量。【结果】肺癌组 织与癌旁组织的端粒酶阳性表达率差异有显著性(P<0.01);肺癌端粒酶活性与淋巴结转移、TNM分期有 显著相关性(P<0.05);肺癌端粒酶活性强度与外周血T淋巴细胞Ag NORs含量呈负相关关系(R=-0. 808)。【结论】端粒酶活化与肺癌的发生发展有关,并与机体免疫功能有着密切的关系,结合分析端粒酶活性 与外周血T淋巴细胞Ag NORs含量对了解肺癌的进展情况有意义。
ObjectiveTo explore the relationship between intensity of telomerase activities and pathological features of lung cancer(LC) as well as argyrophilic nucleolar organizer region proteins (Ag-NORs) content of T lymphocytes in peripheral blood of these patients.The telomerase activities in tissues of 48 cases with LC and para-cancerous tissues of 28 cases were detected by PCR-telomeric repeat amplification protocol (TRAP) approach,. while the Ag-NORs contents in peripheral blood T lymphocytes of 20 patients before operation were estimated by immune mapping analysis.Significant difference in positive telomerase expression rate of LC tissues was found as compared to that of para-cancerous tissues (P<0.01). Telomerase activity in LC was obviously correlated with lymph node metastasis and remote metastasis (TNM) staging (P<0.05); whereas its intensity in LC was negatively correlated with Ag-NORs contents of peripheral blood T lymphocytes (R= -0.844).[Conclusions]Telomerase activation is related to the occurrence and development of LC, and displays a close interconnection with the immunological function of organism. It may be more significant to analyze telomerase activities in combination with Ag-NORs contents of T lymphocytes in peripheral blood during our judgment of LC progression.
出处
《医学临床研究》
CAS
2004年第9期976-978,共3页
Journal of Clinical Research
