摘要
【目的】评价腹水中腺苷脱氨酶 (ADA)和抗结核抗体的检测对鉴别卵巢癌性腹水和结核性腹水的意义。【方法】采用改良的Martineck法和SPA ELISA法对来源于 4 0例卵巢癌患者和 5 0例盆腹腔结核患者腹水中的ADA和抗结核抗体IgG进行了检测。【结果】结核性腹水ADA水平 (75 .4 6U/L)和抗结核抗体阳性率 (84 .0 0 % )显著高于卵巢癌腹水 (分别为 2 2 .73U/L和 12 .5 0 % ;t’ =13.5 87,P <0 .0 1和 χ2 =78.2 71,P <0 .0 0 5 ) ,以ADA >4 5U/L作为阳性标准 ,其对结核性腹水诊断的敏感性、特异性和准确性分别为98.0 0 %、95 .0 0 %和 96 .6 7% ,腹水抗结核抗体对诊断结核性腹水的敏感性、特异性和准确性分别为 94 .2 8%、87.5 0 %和 85 .5 6 %。【结论】腹水ADA水平和抗结核抗体的检测对鉴别卵巢癌腹水和结核性腹水有重要的临床价值 ,腹水ADA水平异常升高和抗结核抗体IgG阳性支持结核性腹水的诊断。
To evaluate the clinical significance of adenosine deaminase (ADA) and anti-TB antibody (ATA) detection in differentiation of ovarian cancerous ascites from tuberculous ascites.The ADA activity and ATA-IgG level in ascitic fluid collected from 40 cases of ovarian cancer and 50 cases of pelvic and/or peritoneal tuberculosis were estimated by improved Martineck's method and SPA-ELISA respectively.ADA activity and positive rate of ATA-IgG in tuberculous ascites (75.46 U/L and 84.00%, respectively) were significantly higher than those in ovarian cancerous ascites (22.73 U/L and 12.50% ) (t' =13.587,P<0.01;χ2=78.271,P< 0.005). As for the diagnosis of tuberculous ascites, when ADA >45 U/L was used as a positive standard , its rates of sensitivity, specificity and accuracy were 98.00%, 95.00% and 96.67% respectively; whereas when ATA-IgG detection was used ,its rates of sensitivity, specificity and accuracy were 94.28%, 87.50% and 85.56% respectively.[Conclusion]Detection of ADA activity and ATA-IgG level shows important significance in differential diagnosis of tuberculous ascites and ovarian cancerous ascites. Abnormal elevation of ADA activity and positive ATA-IgG reaction in ascitic fluid support the diagnosis of tuberculous ascites.
出处
《医学临床研究》
CAS
2004年第6期622-624,共3页
Journal of Clinical Research
关键词
卵巢肿瘤
腹水
结核
腺苷脱氨酶
结核菌素试验
ovarian neoplasms
ascites
tuberculosis
adenosine deaminase
tuberculin test
