摘要
目的评估血清抗结核脂阿拉伯甘露糖-B(LAM-B)和相对分子质量为38-IgG抗体对儿童结核病的诊断价值。方法采用斑点免疫金渗滤法定性检测235例患儿标本[包括血清207份、脑脊液(CSF)43份、胸水8份、腹水5份、胃液4份]中抗结核LAM-B+相对分子质量为38-IgG。结果血清抗LAM-B+相对分子质量为38-IgG阳性率:非结核病组假阳性率1.96%(2/102);结核组为26.67%(28/105),其中肺内结核组32.14%(18/56),肺外结核组20.41%(10/49),两组血清抗体阳性率差异无显著性(P>0.05);菌检阳性合并组抗体阳性率64.00%(16/25)明显高于菌检阴性组抗体阳性率34.29%(12/35),两组有显著性差异(χ2=5.173P<0.05)。结论血清抗LAM-B+相对分子质量为38-IgG检测对儿童结核病有辅助诊断价值。
Objective To evaluate the diagnostic value of antimycobacterial tuberculosis antibody-lipoarabinomannan - B and antigen 5（38 kD） - IgG （ LAM - B ＋ 38 kD- IgG） in children with tuberculosis. Methods LAM - B ＋ 38 kD - IgG in 207 serum sampies,43 cerebrospinal fluid, 8 pleural fluid, 5 ascitic fluid,4 gastric juice specimens were obtained from 235 children with tuberculosis and non-tuberculosis were detected by the dot immanogold filtration assay （DIGFA）. Results The positive rate of LAM - B ＋ 38 kD -IgG in sera was 26.67 % （28/105 ）in tuberculosis group and the false-positive rate of LAM - B ＋ 38kD-IgG in sera was 1.96 % （2/ 102） in non-tuberculosis group. The positive rates of LAM - B ＋ 38kD -IgG in sera in pulmonary tuberculosis group and extrapul- monary tuberculosis were 32.14 % （ 18/56） and 20.41% （ 10/49 ）, respectively, and there was no significant difference between two groups（P 〉0.05）. The positive rate of the antibodies （in sera） in combined- group of bacteriology examination tuberculosis positive results was obviously higher than that of negative group （64.00% vs 34.29% ）. There was significant difference between two groups （χ^2=5. 173 P〈 0.05）. In eases who were smear and culture- negative, 34.29 %cascs tested positive for anti- LAM ＋ 38 kD- IgG. Conclusion These results suggested that the detection of LAM-B ＋ 38 kD-IgG in sera is an useful supplementary tool for diagnosis of tuberculosis in children.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2005年第9期857-859,共3页
Journal of Applied Clinical Pediatrics
