摘要
研究不同水温(18℃和28℃)下,氟苯尼考(Florfenicol,FF)及其代谢物氟苯尼考胺(Florfenicol Amine,FFA)在斑点叉尾体内残留消除规律。以含氟苯尼考4 g/kg的饲料按10 mg/kg鱼体质量连续强饲斑点叉尾(Ictalurus puncta-tus)3 d,于停药后第1天2、天3、天5、天7、天9、天分别将斑点叉尾处死后取肌肉、肝脏、皮肤、肾脏4种组织。采用反相高效液相色谱紫外检测法测定斑点叉尾组织中氟苯尼考及其代谢物氟苯尼考胺。结果表明,不同水温下相同组织,相同水温下不同组织中氟苯尼考及其代谢物氟苯尼考胺的消除速率快慢不一(差异显著P<0.05)。氟苯尼考和氟苯尼考胺之和作为标示残留物的指示值,高水温时标示残留物(总量)在斑点叉尾体内消除的更快。与其他组织相比标示残留物(总量)在肾脏中的消除最慢,因此,建议将肾脏作为标示残留物(总量)在斑点叉尾体内残留的靶组织。在18℃和28℃时,若规定标示残留物(总量)在肾脏中以300μg/kg为最高残留限量(maximum residue limit,MRL),建议休药期分别为234℃.d和224℃.d。本研究旨为不同水温条件下制定该药的休药期提供理论依据。
Florfenicol,a broad spectrum antibiotic,is a fluorinated derivative of thiamphenicol and is also similar in structure to chloramphenicol.Florfenicol has equal or superior antibacterial activity compared to thiamphenicol and chloramphenicol and does not cause human aplastic anemia.Florfenicol is used exclusively in controlling bacteria infectious diseases in cultultued fish.A residue depletion study was conducted of florfenicol(FF) and its metabolite forfenicol amine(FFA) in channel catfish(Ictalurus punctatus) at 18 ℃ and 28 ℃ water temperature.After repeated oral administration at a dosage of 10 mg/kg body weight for 3 consecutive days,channel catfish(Ictalurus punctatus) were killed and the musucle,skin,liver,kindey tissues were collected after oral gavage 1,2,3,5,7,9 d of oral gavage administration.Florfenicol(FF) and florfenicol amine(FFA) in tissues were determination by using reverse phase high performance liquid chromatography(RP-HPLC) with UV detection.The mobile phase was acetonitrile-sodium dihydrogen phosphate solution(0.01 mol/L,containing 0.005 mol/L SDS and 0.10% triethylamine)(2∶3,V∶V) and UV detection set at 225 nm.Analyte were extracted with acetone,Dichloromethane was added that made extracts demixed,Defatted with hexane and so on sample treating process.The standard curves were linear in the investigated ranges,0.05～10.0 μg/g of both florfenicol and florfenicol amine in tissues.The correlation coefficient for florfenicol and florfenicol amine were both r≥0.996 2.The recovery of florfenicol was from 75.14% to 92.65% and the recovery of florfenicol amine was from 76.00% to 91.50%.The within-day and day-to-day precision expressed by CV was less than 10% at two drug levels(0.1 and 1.0 μg/g).The detection limits for florfenicol and florfenicol amine in muscule,skin,liver,kindey were 20 and 15 μg/kg,50 and 50 μg/kg,50 and 50 μg/kg,50 and 50 μg/kg respectively.The results showed that the elimination rate was markedly different in different water temperature and the same tissues or different water temperature and the same tissues.The sum of FF and FFA is taken marker residue.The depletion of the marker residue in channel catfish,Ictalurus punctatus is faster at higher water temperature.To compare with other tissues,the elimination of the marker residue in kindey was slowest,Kindey was the main reservoir of the marker residue in channel catfish(Ictalurus punctatus).It is proposed that withdrawl time were 234 ℃·d and 224 ℃·d respectively at 18 ℃ and 28 ℃,According to the maximum residue limit(MRL) of the marker residue of 300 μg/kg in kindey.The reliable theory basis to make dosage regimen and withdrawl time at different water temperature based on the study.
出处
《中国水产科学》
CAS
CSCD
北大核心
2007年第6期1010-1016,共7页
Journal of Fishery Sciences of China
基金
"十一五"国家科技支撑计划"食品安全关键技术"重大项目(2006BAK02A22)
中国水产科学研究院专项资金项目(2007B003)
