摘要
目的 :研究有氧运动和罗格列酮对NOD(Non -ObeseDiabetic)小鼠PPARγ ,GLUT4和FABPs基因表达的影响。方法 :将 30只NOD小鼠分为 3组 :对照组 (Ⅰ组 )、用药组 (Ⅱ组 ,灌喂罗格列酮 12周 )和运动组 (Ⅲ组 ,进行 12米 /分钟、5 5分钟 /天的跑台训练 12周 )。检测NOD小鼠各项血液生化指标 ,RT -PCR半定量测定肝脏、脂肪和骨骼肌组织PPARγ、GULT4和FABPsmRNA表达。结果 :对照组NOD小鼠血糖显著高于用药组和运动组 (P <0 0 5 ) ,但用药组和运动之间无显著差异。用药组和运动组血脂发生有益变化。与对照组相比 ,用药组和运动组肝脏、骨骼肌和脂肪组织的PPARγ表达增加 1～ 2倍 ,脂肪和骨骼肌GULT4表达均增加 1倍以上 ,脂肪组织A -FABPmRNA水平和骨骼肌H -FABPmRNA水平均高于对照组。结论 :罗格列酮和有氧运动可能通过PPARγ介导GLUT4和FABPs表达上调调节糖脂代谢 ,但是否由PPARγ直接调控GLUT4转录 ,还需要更充分的实验证据的支持。
Objective To study the effects of aerobic exercise and rosiglita zone o n the expression of PPARγ,GLUT4 and FABPs genes. Methods 30 N OD mice were div ided into three groups: controlled group(Ⅰgroup); treated with rosiglitazone fo r 12 weeks(Ⅱgroups);trained in treadmill at speed 12m/min,55min/d for 12 weeks( Ⅲ group).The mRNA expression of PPARγ,GLUT4 and FABPs genes was analyzed by RT -PCR. Results The indexes of serum glucose and lipid showed ben eficial changes in Ⅱ and Ⅲ groups. Compared with the controls, the mRNA expres sion of PPARγ in liver, skeletal muscle and adipose tissue increased significan tly in Ⅱ and Ⅲ groups. The levels of GLUT4 mRNA of the treated groups were hig her than that of the contolled group in skeletal muscle and adipose tissue.Compa red with the controls, the level of A-FABP mRNA in adipose tissue and H-FABP mRN A in skeletal muscle of the treated groups increased. Conclusion Rosiglitazone and aer obic exercise could up-regulate glucose and lipid metabolism via PPARγ expressi on mediated GLUT4 and FABPs expression. Whether PPARγ regulate GLUT4 transcript ion directly needs more experimental evidence.
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2005年第1期55-59,共5页
Chinese Journal of Sports Medicine
基金
国家自然科学基金项目资助
项目号 :30 170 315
