摘要
目的研究金纳多(银杏叶提取物)对大鼠实验性肝纤维化的防治作用。方法应用CCl4诱导大鼠实验性肝纤维化,以金纳多防治。实验分金纳多组(n=12);CCl4组(n=12);正常对照组(n=12)。金纳多组和CCl4组分别用40% CCl4橄榄油溶液制备大鼠肝纤维化模型。金纳多组按8 mL/(kg·d)腹腔注射金纳多液,共12Wk。观察肝脏组织学改变、肝羟脯氨酸(HYP)、丙二醛(MDA)含量、血清PC-Ⅲ、HA、血栓烷B2 (TXB2)及6-酮-前列腺素Fla (6-keto-PGFla)水平。结果金纳多组大鼠肝纤维化程度明显轻于CCl4组;肝羟脯氨酸及丙二醛含量(166.0±27.0μg/g 肝和206.3±1.2μmol/g)显著低于CCl4组(248.0±45.0μg/g 肝和273.5±47.6μg/g);PC-Ⅲ和HA(113.0±42.0μg/L和258.0±92.0μg/L)水平亦显著低于CCl4组(256.0±68.0μg/L和479.0±116.0μg/L),且接近于正常对照组大鼠;TXB2降低,6-keto-PGFla增高。结论金纳多可保护肝细胞、减轻肝细胞坏死,对大鼠实验性肝纤维化具有防治作用。
To study the effects of Ginaton (Ginkgo biloba extract) in prevention of liver fibrosis in mice. The experimental liver fibrosis induced by tetraachloromethone in rats was treated with Ginaton (Ginkgo biloba extract). Wistar rats were divided into three groups: Ginaton group (n =8); CCl4 group (n =8); control group (n =8). Ginaton group and CCl4 group mice were induced by 40% CCl4 olive oil solution into models of liver fibrosis, Ginaton group was injected the Ginaton solution in abdomino (8 ml/kg). Liveer histological changes; hydroxyproline (HYP)and maloldialdehyde (MDA) content in liver tissues; senum PC-Ⅲ, HA, TXB2 and 6-keto-PGF1a levels were observed. The pathlogical studies showed that the liver fibrosis degree of Ginaton group was lighter than that of CCl4 group. Ginaton group has significantly lower level of HYP (166±27 μg/g), MDA (206.3±1.2 umol/g), PC-Ⅲ (113.0±42.0 μg/L) and HA (258.0±92.0 μg/L) than those of CCl4 group (HYP 248±45 μg/g, MDA 273.5±47.6 μg/g, PC-Ⅲ 256.0±68.0 μg/L and HA 479.0±116.0 μg/L, respectively); Ginaton group TXB2 reduce (78.44±32.8 ng/L), CCl4 group (94.5±48.4 ng/L) and 6-keto-PGFla increase (15.1±5.4 ng/L, CCl4 group 12.45±5.48 ng/L). [Conclusion] Ginaton has protection of hepatic cells hepatocellular death and can prevent the formation of liver fibrosis.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2005年第1期67-69,共3页
China Journal of Modern Medicine
关键词
金纳多
肝硬变
疾病模型
Ginaton
liver cirrhosis
disease models
