摘要
目的 :观察银杏叶提取物对大鼠肾缺血再灌注后细胞凋亡的影响。方法 :采用动脉夹闭大鼠双侧肾蒂 4 5 m in、再灌注 2 4 h的方法制成急性肾缺血再灌注模型。光、电镜观察细胞结构改变 ;采用脱氧核苷酸末端转移酶介导的 DNA原位末端标记技术检测细胞凋亡的情况 ,流式细胞仪定量分析细胞凋亡 ;测定血浆肌酐、尿素氮 ,并观察肾功能变化。结果 :缺血再灌注组较假手术组肾小管凋亡细胞数明显增多 ;银杏叶提取物处理组较缺血再灌注组凋亡细胞数明显减少 ,组织损害明显减轻。结论 :银杏叶提取物预防给药能减轻肾缺血再灌注损伤 ,其机制可能与抑制细胞凋亡有关。
Objective: To observe the effects of ginkgo biloba extract(EGb) on apoptosis following renal ischemia/reperfusion injury in rats. Methods: The model of bilateral post-ischemic renal injury by clamping renal pedicles for 45 minutes following 24-hour reperfusion was established. Pathologic and ultrastructural changes were examined by optic and electron microscopy. Apoptosis of tubular cells was assessed by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) method, and the percentage of apoptosis was detected using flow cytometry. The plasma concentrations of creatinine and urea nitrogen were measured for the assessment of renal dysfunction. Results: The damage induced by ischemia/reperfusion was less markedly in EGb pretreated group. Sections of ischemia/reperfusion group showed apoptotic body in hematoxylin and eosin(HE)-stained. The apoptotic cells of uriniferous tubules were increased significantly in ischemia/reperfusion group than those in sham operation group. The TUNEL positive cells and the percentage of apoptotic cell in EGb group decreased significantly (P<0.01), and the tissue damage ameliorated markedly compared with ischemia/reperfusion group. Conclusion: Inhibition of apoptosis may be responsible for the (renal) protective effects of EGb in rats with renal ischemia/reperfusion injury.
出处
《中国中西医结合急救杂志》
CAS
2004年第4期212-214,共3页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
江苏省高校自然科学研究计划项目(03KJB310144)
江苏省徐州科技基金资助项目(2002037)
