摘要
目的观察降脂益肝冲剂对非酒精性脂肪肝大鼠血浆和肝组织血栓素B2(TXB2)和6-酮-前列腺素F1a(6-Keto-PGFla)的影响。方法雄性Wistar大鼠24只随机分为3组(每组8只):正常组给予普通饲料喂养,模型组和治疗组给予高脂饮食喂养。治疗组在实验开始后给予降脂益肝冲剂,同时模型组和正常组分别给予等量的生活饮用水灌胃,8周末处死各组大鼠。苏木素-伊红(HE)染色观察肝脏病理组织学改变,放射免疫法测定血浆和肝组织TXB2和6-Keto-PGFla含量及其比值(T/PG比值)。结果治疗组肝脏脂肪变性程度明显改善,6-Keto-PGFla含量显著高于模型组(P<0.01),TXB2含量、T/PG比值明显低于模型组(P<0.01)。结论降脂益肝冲剂对大鼠血浆和肝组织TXB2、6-Keto-PGFla的含量及其比值具有调节作用,这可能是其抗非酒精性脂肪肝的作用机制之一。
Objective To investigate the effect of Chinese medicine Jiangzhiyigan granula on plasma levels of TXB2 and 6-Keto-PGFla in nonalcoholic fatty liver disease （NAFLD） rats. Methods Twenty four male wistar rats were randomly divided into-three groups（eight rats each） ：normal group fed with standard diet, model group and treated group fed with high-fat diet. The treated group was given Jiangzhiyigan granula at the beginning of experiment. Meanwhile, the normal and model group were given the same quantity of life drinking water respectively. After eight weeks, they were executed death. Their pathologic changes were observed, and the amounts of plasma and liver TXB2, 6-Keto- PGFIa were detected with RIA and the ratio of them was calculated. Results Hepatitosteatosis of the treatment group was obviously improved, the amounts of plasma and liver 6-Keto-PGFla were obviously higher than those in model group, the amounts of plasma and liver TXB2 and the ratio of TXB2/6-Keto-PGFla were obviously lower than that in model group. Conclusion Jiangzhiyigan granula may control the amounts of plasma and liver TXBe, 6-Keto-PGFla and the ratio of TXB2/6-Keto-PGFla, which may be one of the mechanisms of anti-nonalcoholic fatty liver disease.
