摘要
目的 :观察光照对大鼠视网膜氨基酸水平的影响 ,探讨其对视网膜细胞的作用机制。方法 :采用绿色荧光灯持续照射 2 4h造成大鼠视网膜光损伤。分别于光照前、光照后即刻、光照后 14d剥离视网膜 ,测定视网膜游离氨基酸的变化。结果 :除甘氨酸外 ,光照后视网膜天门冬氨酸(ASP)、谷氨酸 (GLU)、γ 氨基丁酸 (GABA)含量升高 ,与正常对照组比较差异有显著性 (P <0 .0 1) ;14d后有所降低 ,但与正常对照组比较 ,差异仍有显著性 (P <0 .0 1)。结论 :急性视网膜光损伤导致视网膜氨基酸类神经递质增高 ,可能是视网膜光感受器细胞凋亡的重要原因。
Objective:To observe the effect of light exposure on the amino acid level in the retina and to study the effect on the cells in the retina.Methods:Sprague Dawley(SD) rats were exposed to green fluorescent light for 24 hours. The levels of aspartate(ASP),glutamate(GLU),glycine(GLY) and γ aminobutyric acid(GABA) in the retina of rats were measured by an amino acid automatic analytical apparatus before light exposure,immediately after exposure,and 14 days after exposure.Results:Compared to the normal group,the levels of ASP,GLU and GABA in the retina immediately after light exposure and 14 days after exposure had increased significantly(P<0.01).Conclusion:An increased level of amino acids in the retina could be an important cause of apoptosis after acute photic injury. [
出处
《眼视光学杂志》
CAS
2004年第4期238-240,共3页
Chinese Journal of Optometry & Ophthalmology
基金
国家中医管理局青年基金课题项目 (3 0 0 5 89)。
