摘要
目的 探讨COX-2、p53和VEGF的表达与胃癌临床病理特征之间的关系,以及COX-2与p53和VEGF表达的关系。方法 胃癌标本84例,采用免疫组化Power vision两步法检测COX-2、p53、VEGF在胃癌中的表达,并分析其临床意义。结果 ①胃癌组织中COX-2的阳性表达率为66.7％(56／84),且COX-2的表达与癌灶大小、淋巴结转移、癌浸润深度和病理类型密切相关(P<0.05或0.01)。②胃癌标本中p53、VEGF的阳性表达率分别为59.5％(50／84)和61.9％(52／84),其表达与癌灶大小、淋巴结转移、癌浸润深度、分化程度和病理类型均无关(P>0.05)。③COX-2阳性胃癌组织p53阳性率(67.86％,38／56)显著高于COX-2阴性者(42.86％,12／28);VEGF表达率在COX-2阳性者为75.00％(42／56),在COX-2阴性者为35.71％(10／28),前者明显高于后者。p53、VEGF均与COX-2表达呈显著正相关(P<0.05)。结论COX-2的表达与胃癌的发生、癌灶大小、淋巴结转移和癌浸润深度密切相关,可作为判断胃癌预后的指标之一;COX-2表达与p53、VEGF之间存在明显的相关性,其机制有待进一步阐明。
Objective To investigate the relationship between cyclooxygenase - 2 (COX - 2) expression and p53 protein and vascular endothelial growth factor ( VEGF) in gastric carcinoma, and to study the significance of them. Methods The expressions of COX - 2, p53 protein and VECF in gastric carcinoma were examined by immunohistochemical method in 84 surgical specimens. Their correlations were further analyzed in connection with the tumor characteristics. Results ① COX - 2 protein was expressed in 56 of the 84 gastric cancers (74% ). The COX - 2 expression was found to be significantly correlated with tumor size, lymph node metastasis, depth of invasion and Lauren's type (P < 0.05 or 0.01) . ② The positive rates of p53 and VEGF expression in gastric carcinoma were 59.5% (50/84)and 61.9% (52/84) , respectively. They were not related to the pathologic characteristics of tumor ( P > 0.05) .③The positive rate of p53 in COX - 2( + ) tumors was 67.86% (38/56) ,significantly higher than that in COX - 2(-) tumors (42.86% or12/28, P<0.05). The positive rate of VEGF was 75.00% (42/56 ) in COX - 2( + ) tumors, but only 35.71 % (10/28) in COX - 2 ( - ) tumors. COX - 2 expression was correlated with both p53 and VEGF expressions (P < 0.05) . Conclusions ①Overexpression of COX - 2 may serve as a prognostic factor to indicate the biological behavior and prognosis of gastric carcinoma. ②Our work provides evidence linking COX - 2 with p53 and VEGF in gastric carcinogenesis, but the mechanism how they interact to promote the neoplasm remains to be elucidated.
出处
《徐州医学院学报》
CAS
2004年第6期498-501,共4页
Acta Academiae Medicinae Xuzhou
基金
江苏省教育厅自然科学基金课题(O1KJB320011)徐州医学院附属医院优秀中青年骨干资助项目
关键词
COX-2
VEGF
胃癌组织
表达
癌浸润
癌灶
淋巴结转移
大小
深度
结论
gastric neoplasms
cyclooxygenase - 2
p53
vascular endothelial growth factor
clinical pathologic characteristics
immunohistochemistry
