摘要
AIM: To investigate the fluid shear stress induced changes of [Ca^2+]i in neutrophils in pancreatic microcirculation of experimental acute pancreatitis (AP).METHODS: Wistar rats (n = 36) were randomized into three groups. A model of AP was established by subcutaneous injection of caerulein. Low-shear 30 viscometer was used to provide steady fluid shear stress on separated neutrophils. The mean fluorescent intensity tested by flow cytometry was used as the indication of [Ca2+]i quantity.RESULTS: Under steady shear, cytosolic [Ca^2+]i showed biphasic changes. The shear rate changed from low to high, [Ca^2+]i in different groups decreased slightly and then increased gradually to a high level (P<0.05). A close correlation was observed between the cytosolic [Ca^2+]i level and the alteration of fluid shear stress in regional microcirculation of AP. CONCLUSION: The increase of [Ca^2+]i is highly related to the activation of neutrophils, which contributes to neutrophil adhesion to endothelium in the early phase of AP. The effect of fluid shear stress on [Ca^2+]i may play a crucial role in pancreatic microcirculatory failure of AP.
AIM:To investigate the fluid shear stress induced changes of[Ca^(2+)]i in neutrophils in pancreatic microcirculation of experimental acute pancreatitis(AP). METHODS:Wistar rats(n=36)were randomized into three groups.A model of AP was established by subcutaneous injection of caerulein.Low-shear 30 viscometer was used to provide steady fluid shear stress on separated neutrophils. The mean fluorescent intensity tested by flow cytometry was used as the indication of[Ca^(2+)]i quantity. RESULTS:Under steady shear,cytosolic[Ca^(2+)]i showed biphasic changes.The shear rate changed from low to high, [Ca^(2+)]i in different groups decreased slightly and then increased gradually to a high level(P<0.05).A close correlation was observed between the cytosolic[Ca^(2+)]i level and the alteration of fluid shear stress in regional microcirculation of AP. CONCLUSION:The increase of[Ca^(2+)]i is highly related to the activation of neutrophils,which contributes to neutrophil adhesion to endothelium in the early phase of AP.The effect of fluid shear stress on[Ca^(2+)]i may play a crucial role in pancreatic microcirculatory failure of AP.
基金
Supported by the National Natural Science Foundation of China,No.39770722 and the Key Project of National Outstanding Youth Foundation of China,No.39925032
