摘要
目的:探讨法舒地尔对急性心肌梗死(AMI)大鼠炎性细胞因子表达的影响及细胞因子表达与Rho激酶的关系。方法:选取雄性Wistar大鼠,建立大鼠AMI模型,将术后存活大鼠随机分为治疗组(F组)和AMI组;另设假手术组(S组),只在其左前降支下穿线不结扎。F组给予法舒地尔5mg/kg,AMI组和S组给予等量0.9%氯化钠溶液,腹腔注射,每日2次。4周后,用Nikon 4多导生理记录仪检测血流动力学指标,放射免疫法测血清中肿瘤坏死因子(TNF)-α和白细胞介素-1β(IL-1β)含量,EvensBlue及NBT双染确定缺血及梗死面积,RT-PCR法测定Rho激酶mRNA的表达。结果:与S组相比,AMI组细胞因子TNF-α和IL-1β、左室舒张末压(LV-EDP)表达明显升高,而左室收缩压(LVSP)和左室压力上升/下降最大速率(±dp/dtmax)明显减低(P<0.05)。与AMI组比较,F组梗死面积显著减小,TNF-α和IL-1β表达水平明显下降,Rho激酶mRNA表达显著减少;F组更明显地降低LVEDP,升高LVSP和±dp/dtmax(P<0.01),左室功能明显改善。结论:法舒地尔可以降低AMI大鼠炎性细胞因子及心肌Rho激酶表达,保护缺血心肌,减少心肌梗死面积,改善心功能。
Objective：To explore the effect of fasudil on the inflammatory cytokine expression in rats heart following acut myocardial infarction（AMI） and its relationship with the Rho kinase.Method：The model of AMI rats were divided into two groups,treated group（F group） and AMI group,besides another group of sham-operation（S group）.Fasudil 5 mg/kg were given into the F group twice a day.The AMI and Sgroup were given equivalent normal saline.Nikon 4 multi-channel physiological recorder records hemodynamics parameters.Radioimmunoassay detected Serum cytokines.EvensBlue and NBT staining identify ischemia and infarction area.The Rho kinase mRNA expression was measured by RT-PCR.Result：Compared with the S group,TNF-α,IL-1β and left ventricular end diastolic pressure（LVEDP） expression in AMI group are significantly higher,the left ventricular systolic pressure（LVSP） and left ventricular pressure rise/fall maximum rate（±dP/dtmax） are significantly lower（P0.05）.Compared with the AMI group,F group significantly reduced infarct size,TNF-α and IL-1β and Rho-kinase mRNA expression levels.In F group,there were significant reduction in LVEDP,increased LVSP and ±dp/dtmax（P0.01）,and better left ventricular function.Conclusion：Fasudil reduced myocardial infarction myocardial Rho kinase and inflammatory cytokine expression,and improved heart function.The effect of Fasudil on inflammatory factors expression and cardiac function may be related to inhibition of Rho kinase activation.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2011年第4期313-316,共4页
Journal of Clinical Cardiology
