摘要
应用免疫组织化学法,观察双侧肾上腺切除后大鼠垂体前叶内降钙素基因相关肽(CGRP)免疫反应神经纤维的变化。将雄性成年大鼠随机分为正常对照组、假手术对照组、补充氢化考的松的正常大鼠组、双侧肾上腺切除补充氢化考的松组及双侧肾上腺切除组。结果显示,正常大鼠垂体前叶内有部分CGRP反应纤维,以细纤维为主,其上有较多膨体;神经纤维多松散走行,有时呈团簇状,其分布不均匀,于内侧偏尾部略多。肾上腺切除组大鼠垂体前叶内CGRP纤维明显增多,包括纤维数目和分支增加、膨体增多增大等;纤维分布广泛。正常大鼠补充氢化考的松后垂体前叶内CGRP反应纤维则有相反变化。在其他各组动物前叶内,未见CGRP纤维有明显变化。计算机辅助图像分析结果显示,肾上腺切除组大鼠垂体前叶内CGRP反应纤维数量显著增多(P<0.05),与补充氢化考的松的正常大鼠垂体前叶CGRP反应纤维比较,差异更显著(P<0.0001);后者与正常对照组结果相比,则显著性减少(P<0.05)。本实验从形态学角度证明,垂体前叶内CGRP纤维可随体内肾上腺皮质激素水平的变化而改变。
Using immunohistochemical method in the present study we examined the changes of the CGRP-LI nerve fibers in the anterior pituitary (AP) of the rat after adrenalectomy. 60 male adult rats were divided into 5 groups at random, i.e., normal, sham-operated, adrenalectomized (AdX) plus hydrocortisone, normal plus hydrocortisone and AdX groups. There were some of CGRP-LI nerve fibers sparsely and unevenly distributed in the normal rat AP. The fibers which had some varicosities and varied in their thickness could occurred either individually or in plexus. The quantity of CGRP-LI nerve fibers increased in number, length, branches and the density of the varicosities. The extent of their distribution was also enlarged. With a Leitz computer-assisted image analyzer the quantity of the CGRP-LI nerve fibers in the 5 groups was measured and the results showed that the amount of CGRP-LI nerve fibers in AdX group was significantly higher than those in all the other groups (P<0.05), while the amount of CGRP-LI nerve fibers in normal plus hydrocortisone group was significantly lower than the other controls (P<0.05), and much significantly lower than that of AdX group (P<0.0001).The results showed that the CGRP-LI nerve fibers in AP varied during changes of adrenocortical hormone status.
出处
《解剖学报》
CAS
CSCD
北大核心
1993年第4期361-367,共7页
Acta Anatomica Sinica
关键词
肾上腺切除
垂体前叶
免疫组织化学
Adrenalectomy
CGRP
Anterior pituitary
Immunohistochemistry
Rat
