摘要
Nanometer-sized fluorescent particles (poly-4-vinylaniline nanoparticles) have been prepared under ultrasonic radiation. The fluorescence of poly-4-vinylaniline nanoparticles (PVN) is ca. 50 folds higher than that of 4-vinylaniline. In comparison with single organic fluorophores, these nanoparticle probes are brighter, more stable against photobleaching, and do not suffer from blinking of light emission. A new fluorimetric method for the deter-mination of reduced glutathione (GSH) has been developed with poly-4-vinylaniline nanoparticles as a probe. The maximal fluorescence for the reagent occurs at excitation and emission wavelengths of 306 and 380 nm, respec-tively. Under optimal conditions, PVN reacts with GSH and o-phthalaldehyde (OPA) to give a highly fluorescent derivative in Na2CO3-HCl buffer (pH=10.55). The fluorescence excitation and emission wavelengths of the fluo-rescent derivative are located at 340 and 428 nm, respectively. The relative fluorescence intensity (RF) is linear in the range of the GSH concentration from 3.0×10-7 to 1.2×10-5 mol/L. The method has been applied to the deter-mination of GSH in synthetic samples with satisfactory results.
Nanometer-sized fluorescent particles (poly-4-vinylaniline nanoparticles) have been prepared under ultrasonic radiation. The fluorescence of poly-4-vinylaniline nanoparticles (PVN) is ca. 50 folds higher than that of 4-vinylaniline. In comparison with single organic fluorophores, these nanoparticle probes are brighter, more stable against photobleaching, and do not suffer from blinking of light emission. A new fluorimetric method for the deter-mination of reduced glutathione (GSH) has been developed with poly-4-vinylaniline nanoparticles as a probe. The maximal fluorescence for the reagent occurs at excitation and emission wavelengths of 306 and 380 nm, respec-tively. Under optimal conditions, PVN reacts with GSH and o-phthalaldehyde (OPA) to give a highly fluorescent derivative in Na2CO3-HCl buffer (pH=10.55). The fluorescence excitation and emission wavelengths of the fluo-rescent derivative are located at 340 and 428 nm, respectively. The relative fluorescence intensity (RF) is linear in the range of the GSH concentration from 3.0×10-7 to 1.2×10-5 mol/L. The method has been applied to the deter-mination of GSH in synthetic samples with satisfactory results.
基金
Project supported by the Education Young Teacher Foundation of Anhui Province (No. 2004jq121)
the National Natural Science Foundation of China (No. 20375001) and the Natural Science Foundation of Anhui Province (No. 03044904).
