摘要
目的:建立测定积雪草配方颗粒剂中积雪草苷的HPLC含量分析方法。方法:采用C18柱,RP-HPLC法,水-乙腈为流动相,梯度洗脱,检测波长205 nm。结果:积雪草苷在0.02-0.51 mg·mL-1范围内,峰面积与浓度呈线性关系,r=0.9999,高、中、低浓度的平均回收率分别为99.6%,101.7%,102.2%;RSD分别为2.9%,1.7%,4.9%(n=3)。结论:该方法简便,结果准确,适用于积雪草配方颗粒剂的质量控制。
Objective ;To establish a method which can determine the assiaticoside in formula particles of Centella asiatica L. Methods;Using C18 reversed -phase column,mobile phase as acetonitrile -water with gradient elution, detection wavelength at 205 nm. Results;The calibration curve was linear in the range of 0. 0204 -0. 51 mg · mL-1 for assiaticoside. The average recovery of assiaticoside were 99. 6% ,101. 7% and 102. 2% respectively with corresponding RSD of 2. 9% ,1.7% and 4. 9% (n =3). Conclutions:The method was simple and accurate for quality control of the Formula particles of Centella asiatica L..
出处
《药物分析杂志》
CAS
CSCD
北大核心
2004年第5期516-518,共3页
Chinese Journal of Pharmaceutical Analysis
基金
上海市科委科技攻关项目
色谱和信息处理联用作中药指纹图谱鉴别及其鉴别库(01DJ19012)
