摘要
目的 研究大柴胡颗粒的体外抑菌和体内抗菌效果。方法 测定大柴胡颗粒在体外的最低抑菌浓度(MIC)和最低杀菌浓度 (MBC)。又以金黄色葡萄球菌 (ATCC2 5 92 3)感染小鼠 ,口服大柴胡颗粒以判断其体内抗菌效果 ,并计算其半数有效量 (ED50 )。结果 大柴胡颗粒对金黄色葡萄球菌等 10种常见致病菌有较强的体外抑菌作用。口服一定剂量大柴胡颗粒的感染小鼠生存率明显高于阴性对照 ,其对小鼠的ED50 为 9 6 0 g/(kg·d) ,95 %置信区间为 6 5 7～ 14 0 2 g/(kg·d)。结论 大柴胡颗粒的体内、外抗菌作用均明显 ,此实验结果为其临床应用提供了药效学依据。
Objective To study the antibacterial effect of Dachaihu (DCH) granulation in vitro and in vivo. Methods Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of DCH granulation were determined in vitro. Mice were given different doses of DCH granulation (per os), and the antibacterial effect on the mice infected by staphylococcus aureus (ATCC25923) was observed. Results DCH granulation had strong inhibitory effect against the 10 common clinical pathogens in vitro. The section in that mice were given certain dose of DCH granulation had higher survival rate than the negative control. The ED_ 50 was 9.60 g/(kg·d), and the 95% confidence interval was 6.57-14.02 g/(kg·d). Conclusion DCH granulation has significant antibacterial effect in vitro and in vivo. The results provide a basis for clinical application of DCH.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2004年第5期619-621,共3页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
