摘要
在体外用细胞培养的方法,将人脐带静脉内皮细胞种植在已培养成复层的人脐带动脉平滑肌细胞上面,人工组建成动脉壁结构。在营养液中含内皮细胞生长因子及维生素C条件下,两种细胞生长良好,构成活的具有代谢过程及增殖力的血管壁。光镜及电镜下观察,两种细胞组成典型的血管壁的结构层次,在内皮细胞与平滑肌细胞之间,在平滑肌细胞与平滑肌细胞之间,充填有大量胶原纤维、网状纤维与少量的弹力纤维,并发现这些纤维结构物质是由血管平滑肌细胞产生的。为研究血管壁的生理与病理过程提供了良好的血管壁模型。
Using cell culture technique, in vitro an artificial arterial wall has been constructed by adding cultured endothelial cells derived from the human umbilical vein to the smooth muscle cells isolated from the human umbilical artery. On the condition of vit C and the endothelial cell growth factor in the medium, both the endothelial cells and smooth muscle cells grew very well and formed living arterial walls with the ability of generation and metabolism. A confluent monolayer of flat endothelial cells and multilayer of smooth muscle cells were observed under the light and electron microscope. Among these cells were plenty of collagenous fiber, elastic fiber and glycoproteins produced by the smooth muscle cells. This blood vessel wall model can be used for studying the physiological and pathological process of arteries.
出处
《昆明医学院学报》
1989年第4期1-5,共5页
Journal of Kunming Medical College
基金
京港学术中心资助
关键词
动脉壁
细胞培养
电镜
Endothelial cell
Smooth muscle cell
CoUagenous fiber
Arterial wall
