摘要
目的 对患者体内分离出的真菌进行鉴别诊断。明确马尔尼菲青霉菌与荚膜组织胞浆菌的主要区别。方法 取患者血液、骨髓、皮损分泌物等接种于沙保罗培养基 ,分别置 2 5℃和 37℃孵育 ,观察菌体生长情况及菌落形态 ,并涂片、固定、染色 ,置显微镜下观察菌体特征。结果 从患者体内分离出双相性真菌 ,于 2 5℃为霉菌相 ,在含糖培养基中产生特殊的红色色素 ,镜下可见典型的帚状枝 ;于 37℃为酵母相 ,无色素产生 ,并可见腊肠状细胞和横壁。可排除荚膜组织胞浆菌 ,鉴定为马尔尼菲青霉菌。结论 马尔尼菲青霉菌与荚膜组织胞浆菌诊断的金标准是从体内分离出病原菌 。
Objective The fungus which separated from pati en t was identificated.To clarify the main distinction between penicillium marnef fei and histoplasma capsulatum.Methods The bone mar row,blood and secretion of dermatic lesion were inoculated on Sabouraud's agar,a nd incubated at 25 ℃ and 37 ℃ respectively.The growth and colony of the fungus were inspected and the characteristics of the mold’s smear were observed under microscope.Results A kind of dimorphic fungus was separa ted from the patient.It appeared to be mold type at 25 ℃ and generated a charac teristic soluble red pigment in surgery medium and presented typical broom-shap ed under microscope.It appeared yeast type at 37 ℃ without pigment yielded and presented denticle macroconidiumia sausage-shaped under microscope.Accordingly histoplasma capsulatum was excluded and penicillium marneffei was identi ficated.Conclusion The diagnostic criteria of penicilli osis and histoplasmosis depend on the respective pathogen separated from p atients.The characteristic identification of fungus was very important to diagno se the two similar disease.
出处
《现代检验医学杂志》
CAS
2004年第5期12-13,共2页
Journal of Modern Laboratory Medicine
关键词
马尔尼菲青霉菌
荚膜组织胞浆菌
鉴别诊断
penicillium marneffei
histoplasma capsulatum
differential diagnosis
