摘要
为研究JDV与其它三种牛反转录病毒BIV、BLV、BFV的相互作用关系,将以JDV、BIV、BLV、BFV的LTR为启动子,以Luc为报告基因的质粒和以上病毒反式激活因子的表达质粒共转染BL12细胞系,通过瞬时表达分析试验证明了JDV和BIV的LTR和Tat之间亲缘关系很近,能够相互激活;JDVTat可以反式激活BLVLTR,BLVTax不能激活JDVLTR;JDVLTR上存在BFVTas的应答元件;BLV、BFV和BIV的LTR和反式激活因子间不存在相互激活。
To study the interaction among Jembrana disease virus, Bovine immunodeficiency virus, Bovine Foamy Virus and Bovine Leukemia Virus, a series of plasmids were constructed with their LTR and luc report gene, respectively, and transactivator expression plasmids of these four viruses were also constructed. These plasmids were co-transfected into BL12 cell line, respectively. Transient assay showed the interactivation between LTR and Tat of BIV and JDV, suggesting that they could be transactivated each other. In addition, BFV Tas could transactivate JDV LTR and JDV Tat could transactivate BLV LTR whereas BLV Tax had no effect on JDV LTR and no other interactivation was detected among BLV, BFV and BIV.
出处
《中国病毒学》
CAS
CSCD
2004年第5期471-475,共5页
Virologica Sinica
基金
国家自然科学基金(30270059)
