摘要
扩增并克隆了ＪＤ病毒的ｇａｇ 基因片段（位于３００ｎｔ～５６４ｎｔ，编码基质蛋白ＭＡ）和ｐｏｌ基因片段（位于３４６７ｎｔ～３６９１ｎｔ，编码反转录酶ＲＴ），测定其序列并同ＢＩＶ、ＢＦＶ 和ＢＬＶ 的相应基因进行比较．所建立的方法能够特异性扩增ＪＤＶ序列。
Two fragments from Jembrana disease virus (JDV) genome were amplified by PCR. The first located in 300nt to 564nt region of gag gene, encoding viral matrix protein (MA). The second located in 3467nt to 3691nt region of pol gene, encoding the reverse transcriptase (RT). The fragments were cloned into pUC18 plasmid and confirmed by DNA sequencing, then were compared with gag and pol genes of bovine immunodeficiency virus (BIV), bovine foamy virus (BFV), and bovine leukemia virus (BLV). It can be used to detect the JDV provirus by PCR analysis as described.
出处
《南开大学学报（自然科学版）》
CAS
CSCD
北大核心
1999年第3期151-154,共4页
Acta Scientiarum Naturalium Universitatis Nankaiensis
基金
天津市自然科学基金
关键词
JD病毒
序列分析
基因组
克隆
牛病毒
慢病毒
Jembrana disease virus (JDV)
polymerase chain reaction (PCR)
sequence analysis
