摘要
目的 观察糖尿病大鼠认知能力及海马内神经元生长抑素基因表达产物mRNA改变 ,研究糖尿病对海马神经元内生长抑素表达的影响 ,探讨慢性糖尿病脑病的发病机制。方法 Wistar大鼠通过腹腔注射链脲佐菌素建立糖尿病大鼠模型。Y型电迷宫法测定认知能力 ,原位杂交组织化学技术和计算机图像分析技术检测和比较糖尿病大鼠与正常大鼠海马神经元内生长抑素mRNA的表达。结果 糖尿病大鼠认知能力下降 ,海马生长抑素mRNA阳性神经元细胞数量、阳性细胞的光密度及阳性细胞平均细胞面积均比正常大鼠显著减少。结论 糖尿病是老年性痴呆的高危因素 ,糖尿病大鼠海马神经元的生长抑素表达减少 。
Objective To investigate the ralationship between diabetes and somatostatin(SOM).Methods We established a streptozocin-induced diabetic rats model and stutied the changes of SOM mRNA in hippocampi of diabetic and normal rats by using in situ hybridication and computer image analysis.We studied the machanism of chronic diabetes encephalopathy by observing the changes of somatoostatin mRNA in the hippocampus of diabetic rats was.Results The number,light density and average area of positive cells in dentate gyrus area of hippocampus in diabetes model were reduced significantly as compared with normal rats (P<0 01).Conclusion A decline in SOM mRNA expression may play a role in chronic diabetes encephalopathy.
出处
《湖北民族学院学报（医学版）》
2004年第3期1-3,共3页
Journal of Hubei Minzu University(Medical Edition)
关键词
生长抑素
海马
糖尿病
大鼠
somatostatin
hippocampus
diabetes
rat
