摘要
探讨人外周血单个核细胞(PBMC)在丝裂原刺激后产生白细胞介素-2(IL_2)和干扰素(IFN_s)的关系。PHA、SPA、ConA、PolyI:C均可诱导IL_2的产生。如联合采用PolyI:C+PHA、PolyI:C+ConA在IL_2的诱生中有明显诱生协同作用。IFN-α+PHA、IFN-γ+PHA对IL_2的诱生也能明显提高效应。IL_2还能明显增强IFN-α和IFN-γ的生成。若将IL_2+PHA联合刺激可加强IFN-γ生成作用,IL_2+NDV联合刺激可提高IFN-α的生成。
This paper reports that all the IFN inducing agents including PHA, ConA, SPA,PloyI: C can induce the pr duction of IL_2 by human periphery blood mononucleocytes invitro. Both the combination of PolyI: C+ConA; PolyI: C+PHA, and the combination ofIFNα+PHA; and IFN_γ+PHA show synergistic effect on the production of IL_2·Besides,IL_2 enhanced the production of IFN_γ and IFNα. To stimulate human PBM, the combinationof IL_2+PHA enhanced IFN_γ production in vitro as well as the combination of IL_2+NDVenhanced IFNα production.
