摘要
为探讨白细胞介素与癫痫发病机理的关系，该研究应用脑电图仪记录了侧脑室内注射白细胞介素－２（ＩＬ－２）后，大鼠海马脑电图的变化。结果显示：侧脑室内注射ＩＬ－２后，大鼠海马脑电图出现阵发性痫性放电，表现为频发棘波；应用免疫组织化学方法（ＰＡＰ法）和显微图像分析技术，观察到：侧脑室内注射ＩＬ－２后，大鼠海马回和齿状回内胶质原纤维酸性蛋白（ＧＦＡＰ）免疫反应的胶质细胞的数量明显增多、胞体截面积增大、突起及其分支增多。对大鼠海马回ＣＡ１区ＧＦＡＰ免疫反应的胶质细胞进行显微图像分析，结果显示；与正常对照组相比，ＩＬ－２组的细胞数量约增加０．４８倍、胞体截面积约增加１．７３倍、周长约增加１．０９倍、平均光密度约增加０．２０倍、积分光密度约增加２．３１倍。统计结果表明：差异有极显著性意义（Ｐ＜０．０１）。上述结果提示：大鼠侧脑室内注射ＩＬ－２可促进海马星形胶质细胞的增殖、肥大和细胞突起的萌芽。
In order to explore the relation between interleukin and the pathogenesis of epilepsy,the electroencephalogram(EEG) equipment was applied to record the EEG changes of the rat hippocampus after injecting interleukin 2(IL 2 4μl,5000U/L) into the lateral ventricle.The result suggested that the EEG of the rat hippocapus showed epileptic discharges,which appears to be frequent spike wave.The technique of innumohistochemisty(PAP) and microphotographic analyses were applied to observe and detect the changes of glial fibrillary acidic protein(GFAP)immuno reaction positive glial cells in the rat hippocampal gyrus and dentate gyrus after injecting IL 2 into the lateral ventricle.For the first time we observed that GFAP immuno reaction positive glial cells of hippocampal gyrus and dentate gyrus increased in areas of cross section,processes and its branches after injecting IL 2 into the lateral ventricle.Microphotographic analyses of GFAP immuno reaction positive glial cells of CA 1 region of rat hippocampal gyrus showed that number of cell imcrased 0 48 times,areas of cross section increased 1 73 times,perimeters increased 1 09 times,Means of optical density increased 0 20 times and integration of optical density increased 2 31 times,comparing with those in normal rat.Statistical results showed a signigicantly difference( P <0 01).The results mentioned above suggested that IL 2 injecting into lateral ventricle might promote proliferation,hypertrophy,germination of astrocytes.
出处
《解剖学报》
CAS
CSCD
北大核心
1998年第1期21-26,I002,共6页
Acta Anatomica Sinica
基金
国家自然科学基金
关键词
白细胞介素2
海马
癫痫
脑电图
胶质细胞
Interleukin 2
Hippocampus
Glial fibrillary acidic protein
Glial cell
Immmunohistochemisty
Microphotographic analyses
