摘要
目的 探讨蛋白激酶C (proteinkinaseC ,PKC)在鼻息肉组织中嗜酸粒细胞 (eosinophlis,EOS)浸润增多的意义。方法 采用原位杂交和免疫组化法 ,对 2 6例鼻息肉组织和 2 0例下鼻甲黏膜进行PKC和促细胞凋亡基因Bax、抑凋亡基因B细胞淋巴瘤、白血病 2 (B celllymphoma leukemia 2 ,Bcl 2 )进行检测 ;用May Gr櫣ｎｗａｌｄ Giemsa(MGG)常规染色鉴别EOS。结果 ①EOS中Bcl 2mRNA及其蛋白的阳性细胞表达率鼻息肉组均高于下鼻甲组 (P <0 0 1) ,而鼻息肉组EOS中BaxmRNA及其蛋白的阳性细胞的表达率均略高于下鼻甲组 ,但差异不显著 (P >0 0 5 ) ;②鼻息肉组 2 6例EOS中均有PKC表达 ,而下鼻甲组EOS只有 7例偶有PKC表达。 2组标本EOS中PKC的表达率差异有显著性。且鼻息肉组嗜酸细胞中PKC与Bcl 2mRNA及其蛋白的表达分别呈明显的正相关 (r1 =0 0 875 ,r2 =0 0 82 3,P <0 0 1)。结论 鼻息肉EOS中PKC表达增多与凋亡抑制密切相关 。
Objective To explore the significance of protein kinase C (PKC) in eosinophil(EOS)proliferation and infiltration in nasal polyp tissues Methods With the methods of in situ hybridization and immunohistochemistry staining, PKC and apoptosis gene (Bcl 2, Bax )expression were measured between nasal polyps from 26 patients and inferior turbinate mucous membrane tissue (ITMMT) from 20 normal persons EOS was identified with May Grünwald Giemsa (MGG) staining Results In EOS, the positive cell expressive rate of Bcl 2 mRNA and its protein were significantly higher in group of nasal polyps than that in group of ITMMT ( P <0 01) Although the positive cell expressive rate of Bax mRNA and its protein were slightly higher in group of nasal polyp tissue than that in group of ITMMT ,the difference was not significant ( P >0 05) PKC was expressed in EOS of all 26 cases from nasal polyp tissues, but in 20 cases from ITMMT, only 7 cases showed occasional PKC expression In both groups, PKC positive cell expression was significantly different, and the expression of PKC and Bcl 2 mRNA as well as their protein in EOS of nasal polyps showed remarkably positive relation ( r 1= 0 087 5, r 2= 0 082 3, P <0 01) Conclusion PKC expression increasing in EOS of nasal polyp tissues was closely associated with apoptosis inhibiting So we presumed that EOS inhibiting in nasal polyp tissues was obtained by activating PKC signal transduction pathway
出处
《中华耳鼻咽喉科杂志》
CAS
CSCD
北大核心
2003年第2期83-85,I001,共4页
Chinese Journal of Otorhinolaryngology
基金
吉林大学创新基金 (4 0 3 0 10 12 3 15 4)
