摘要
目的探讨铬 (Ⅵ )与还原型谷胱甘肽 (GSH)之间的作用机制 ,并建立一种GSH的电化学检测方法。方法采用循环伏安法研究GSH与铬 (Ⅵ )相互作用 ,从而影响铬 (Ⅵ )在金电极上的电响应。结果在含 0 .0 0 1mol/LH2 SO4的 0 .1mol/LNaNO3 溶液中铬 (Ⅵ )离子在金电极表面于 +0 .2V(Vs .SCE)有一较强的还原峰 ,当溶液中加入少量GSH时 ,该峰电流下降 ,并且随着GSH的不断加入峰电流逐渐减小 ,在一定的浓度范围内GSH加入量与峰电流的下降呈线性关系 ,据此建立了一种GSH的电化学检测方法 ,其线性范围为 7.0× 10 -8～ 1.0× 10 -9mol/L ,检测限为 1.0× 10 -9mol/L ,RSD为 0 .4 %。结论获得灵敏、简便、快速的间接的GSH电化学分析方法。
PurposeTo investigate the interaction of glutathione GSH and chromium (Ⅵ), and to establish a method of determinating GSH.MethodsGSH reduced the current of chromium (Ⅵ) by using cyclic voltammetry.ResultsA strong reduced peak appeared at about 0.2 V in 0.1 mol/L NaNO 3 0.001 mol/L H 2SO 4 on a gold electrode. When GSH was added in the solution,the reduced peak current was depressed.The results showed that the reduced peak current of chromium (Ⅵ) had good linearity with concentration of GSH in the range of 1.0×10 -9～7.0×10 -8 moL/L, and the detection limit was 1.0×10 -9 moL/L. ConclusionA simple,sensitive and fast electrochemical analysis of GSH was introduced.
出处
《中国生化药物杂志》
CAS
CSCD
2004年第5期273-275,296,共4页
Chinese Journal of Biochemical Pharmaceutics
基金
广东省自然科学基金资助项目 (No .0 2 1190 )
广州市科技项目 ( 2 0 0 3Z D2 0 41)
关键词
谷胱甘肽
铬(Ⅵ)
电化学检测
glutathione
chromium(Ⅵ)
electrochemical defection
