摘要
本文研究了铜 ( ) -新铜试剂 -谷胱甘肽 -乙醇体系的显色反应 ,探讨了反应条件。试验中发现乙醇可以使吸光度增加 13.8% ,提高了显色反应的灵敏度。该方法用于测定大豆提取液中谷胱甘肽的含量 ,取得了满意结果。
The colour reaction of glutathione with copper ( Ⅱ)-neocuproine -alcohol was investigated in the present paper.The absorbance increased distinctly by about 13.8% when alcohol was added to the system.The ma ximum absorption wavelength λ max of [Cu(neocuproine) 2]- + was at 456 nm and the apparent molar absorptivity was 1.2×10-4 L·mol- -1·cm- -1 in the selected system.Linear dynamic relationship was obta ined in the concentration range of 2—24 μg·mL- -1 for glutathione.Th e regression equation was as following:A=0.010 4+0.038 5c.The correlat ion coefficients was 0.999 8.The recoveries obtained were between 98.5% and 99 .9%.The relative standard deviations were less than 0.9% for all analyses.This method was used to determine glutathione in soybean extracts by the electromagn etic field technique and satisfactory results were obtained.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2000年第3期412-414,共3页
Spectroscopy and Spectral Analysis
基金
吉林省卫生厅科研基金资助项目
