摘要
谷胱甘肽（ＧＳＨ）是一种内源性放射防护物质，在正常状况下大部分以还原型存在于细胞和组织中。本研究结果表明，通过外源性化学修饰剂丁胱亚磺酰亚胺（ＢＳＯ），可有效地抑制细胞内ＧＳＨ的生物合成，从而使内源性ＧＳＨ含量下降，在１ｍｍｏｌ／ＬＢＳＯ作用后２４ｈ，可使原有的ＧＳＨ含量从４．６７６８４×１０－１２ｍｍｏｌ／ｃｅｌｌ下降至０．０５０９２×１０－１２ｍｍｏｌ／ｃｅｌｌ，为对照组的１．００％。ＢＳＯ的抑制作用与其浓度和作用时间长短有关：０．１ｍｍｏｌ／ＬＢＳＯ作用２ｈ后，ＧＳＨ含量为２．６３４４８×１０－１２ｍｍｏｌ／ｃｅｌｌ，而在作用４８ｈ后，ＧＳＨ含量为０．０９２７７×１０－１２ｍｍｏｌ／ｃｅｌｌ。即ＢＳＯ在０．１ｍｍｏｌ／Ｌ浓度时，作用４８ｈ后细胞内ＧＳＨ下降为对照组的１．９８％。细胞在ＧＳＨ含量降低的情况下，进行电离辐射，通过对细胞放射增敏比（ＳＥＲ）的观察表明，ＧＳＨ下降时ＳＥＲ值增大，说明减少ＧＳＨ的含量，可增加细胞对射线的敏感性。这对放射生物学和临床放射肿瘤学的理论与实践均有一定意义。
The role of intracellar glutathione (GSH) in radiation protection has been noticed in recent years. In this study, we found that the level of intracellar glutathione and radiosensitization had a certain relationship. It was recognized that the higher GSH level, the lower radiosensitization. The GSH level in V 79-379 A, Y 79. SPC-A-1. SGC-7901 and So-Rb 50 cells were showed in this experiments by Tietze method. The GSH level which was depleted by BSO was assessed. The radiosensitization was determined by the standard colony formation assay iN VITRO.The results showed that SER (Sensitization Enhancement Ratio) for V 79-379 A cell was increased with GSH depletion. The SER for BSO 0. 1. 0. 5 and 1. 0 mmol/Lwere 1. 16, 1. 34 and 1. 50, respectively. Same results for Y 79 cells were also observed that the SER for BSO 0. 01 and 0. 05 mmol/L were 1. 21 and 1. 36 respedively.On the other hand, the exposure time of BSO also influenced GSH level and SER.These results suggested that the level of glutathione in cell and radiosensitization has a close relationship.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
北大核心
1996年第3期172-176,共5页
Journal of Radiation Research and Radiation Processing
基金
国家自然科学基金
关键词
谷胱甘肽
放射敏感性
Glutathione (GSH), Buthione sulfoximine (BSO), Radiosensization
