摘要
目的 :观察银杏内酯 (GL )对大鼠脑缺血再灌注损伤的保护作用。方法 :采用大脑中动脉栓塞法制作大鼠急性脑缺血再灌注损伤的模型。缺血 1h后灌胃给药或生理盐水 ,继续缺血 1h后再灌注 2 4 h,观察再灌注后大鼠的神经功能损害改变及评分 ;取实验鼠大脑组织测定脑含水量 ;用质量分数为 2 %的红四氮唑染料(TTC)染色以测量脑梗死体积。结果 :大鼠急性脑缺血再灌注后神经功能损害评分在 GL 各剂量组均明显低于对照组 (P均 <0 .0 1) ;GL 中剂量 (49.5 m g/ kg)和高剂量 (14 8.5 mg/ kg)均可显著降低缺血再灌注损伤大鼠脑组织含水量 ,减轻缺血侧脑半球水肿程度 ;GL各剂量组均能显著缩小缺血再灌注一侧脑组织梗死体积。结论 :GL对脑缺血再灌注引起的脑损伤具有明显的保护作用。
Objective:To study the cerebral protective effects of Ginkgolide(银杏内酯) on cerebral ischemia reperfusion injury in rats.Methods:The model of acute reperfusion injury after cerebral ischemia in rats was made by middle cerebral artery occlusion.One hour after the ischemia occurred,the Ginkgolide and saline were via gastric garage to the rats respectively and the ischemic condition lasted for another hour with a following 24 hours reperfusion.The neurological grades and the cerebral water were measured.The volume of the cerebral infarction by 2% TTC stained was estimated.Results:The neurological grades with acute reperfusion injury after cerebral ischemia in any of the Ginkgolide groups were significantly lower than that in the control group( all P <0 01 ).In Ginkgolide middle dose (49 5 mg/kg) and high dose (148 5 mg/kg) groups,Ginkgolide could significantly decrease the cerebral water content,and relieve edema.Ginkgolide of all doses could reduce the volume of cerebral infarction of the impaired brain hemisphere in a dosedependent manner.Conclusion:Ginkgolide has great cerebral protective effects against ischemiareperfusion injury.
出处
《中国中西医结合急救杂志》
CAS
2003年第1期31-33,共3页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
广东省科技计划项目资助 (2 KB0 42 0 7S)
