摘要
目的 通过研究环氧合酶 2 (COX 2 )在胃癌、癌前病变及慢性炎症中的表达情况 ,探讨COX 2与胃癌发生、发展的关系及意义。方法 采用免疫组化和原位分子杂交法 ,对 15例正常胃黏膜组织及 12 3例不同病变胃黏膜组织标本中COX 2进行检测。结果 COX 2蛋白在胃癌组织中的表达明显增强 ,其阳性率 (6 2 .2 6 % )显著高于正常胃黏膜、慢性浅表性胃炎、慢性萎缩性胃炎伴肠化及不典型增生胃黏膜组织 (6 .6 7% ,17.86 % ,30 .4 3% ,4 2 .11% ;P均 <0 .0 5 ) ;COX 2蛋白的阳性表达与胃癌组织学分级、浸润深度及有无淋巴结转移有关 ;高、中分化胃癌组中COX 2的表达明显高于低分化组 ;同时 ,在COX 2蛋白阳性的相关胃黏膜组织细胞中亦可见到COX 2mRNA的阳性表达。结论 COX 2的过度表达可能参与了胃癌形成的全过程 ,不仅在胃癌形成的早期 ,且与胃癌的侵袭、转移及预后亦有一定关系。
Objective To investigate the expression of cyclooxygenase-2(COX-2) in human gastric carcinoma,precarcinoma changes,chronic inflammation and to identify the significance of COX-2 expression.Methods COX-2 protein and mRNA were detected in 15 normal gastric mucosa(NGM),123 various gastric changes tissue specimens by using immunohistochemistry and in situ hybridization.Results There was a significantly much higher positive rate( 62.26% ) of cox-2 protein in gastric carcinoma(GC) than that in NGM,chronic superficial gastritis(CSG),chronic atrophic gastritis with intestinal metaplasia(CAG+IM) and atypical hyperplasia(Dys) tissues( 6.67% , 17.86% , 30.43% , 42.11% ;P< 0.05 respectively); The overexpression of COX-2 in GC was correlated with histological grade,invasive depth and lymph node metastasis.The higher the differentiation of gastric carcinoma,the more the expression of COX-2.At the same time,the expression of COX-2 mRNA was mostly observed in the related cells of gastric tissues with positive COX-2 protein.Conclusion The overexpression of COX-2 might participate in the whole process of the occurrence and development of gastric carcinoma,and relate to early dignosis or poor prognosis.
出处
《临床荟萃》
CAS
北大核心
2004年第14期784-786,共3页
Clinical Focus
关键词
胃癌
环氧合酶-2
免疫组织化学
原位分子杂交
gastric carcinoma
cyclooxygenase-2
immunohistochemistry
in situ hybridization
