摘要
目的 探讨创伤合并感染对机体肠屏障功能的损害作用。方法 随机、对照性实验。雄性成年SD大鼠 3 5只 ,分为对照组 (Con)及肠内营养 (EN)、静脉营养 (PN)和脓血症组 (Sep)。后三组做成静脉营养模型 ,施以不同损害刺激。结果 Sep组小肠黏膜损害最重 ,内毒素导致EN、Sep组血浆中一氧化氮升高 ,分别为 10 3 .3 9、113 .3 3 μmol/L ;Sep组小肠通透性和细菌易位率增加 ,分别为 4.0 0 %和 83 .3 3 %。EN组肠屏障损害显著减轻。结论 静脉营养 1周导致大鼠小肠黏膜萎缩 ,肠通透性增加 ;内毒素加重了创伤大鼠肠屏障功能损害 ,可能与体内一氧化氮产生增多有关 ;
Objective To investigate function damage of the intestinal barrier by trauma and infection in rats.Methods The randomized and controlled experiments that mimic surgical trauma and infection in rats.Adult Sprague Dawley rats were divided into four groups:control group ( n =8),EN group ( n =10),PN group ( n =9) and Sep group ( n =8).The latter three groups received different harmful stimulations.Results The inflammatory responses became gradually aggravated from group EN to Sep group.The mucosal structure of small intestine was markedly impaired in groups PN and Sep.Lipopolysaccharide injection increased the nitric oxide levels in the blood plasma of the rats,they were 103.39 umol/L in EN group and 113.33 μmol/L in Sep group..The intestinal permeability was 4.0088 and bacterial translocation rate was 83.33% in Sep group,they all increased significantly than that of control group.Conclusion Parenteral nutrition for one week caused an atrophy of the intestinal mucosa in rats.Endotoxemia (Lipopolysaccharide) damaged the intestinal barrier function due to the production of nitric oxide in the body,and enteral nutrition decreased the kind of damage.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2004年第6期697-699,共3页
Chinese Journal of Experimental Surgery
关键词
创伤
内毒素
大鼠
肠屏障功能
损害作用
Endotoxemia
Parenteral nutrition
Intestinal barrier function
Bacterial translocation
Lactulose/Mannitol test
