摘要
目的观察大鼠内毒素血症早期枯否细胞(KC)在小肠黏膜损害中的作用及氯化钆(GdCl3)阻断KC功能后对肠道完整性的影响。方法将大鼠分为3组。A组:单纯注入内毒素;B组注入内毒素之前24h先经静脉注入GdCl3;C组:假手术对照组。注射内毒素后4h处死大鼠,取材并收集胆汁。光镜下观察回肠黏膜的形态学变化。分离大鼠的KC,用RT-PCR检测KC中肿瘤坏死因子-α(TNF-α)和白细胞介素6(IL-6)mRNA的表达。采用ELISA检测胆汁和血浆中TNF-α和IL-6水平的变化。结果A组回肠黏膜绒毛上皮表浅坏死,伴中性粒细胞浸润以及上皮脱落;B组回肠黏膜损害明显减少;C组回肠黏膜的形态学无改变。A组KC表达TNF-α和IL-6mRNA显著;B组表达明显减少;C组表达不明显。A组胆汁中TNF-α和IL-6的水平分别为(1032±107)pg/ml和(1185±127pg/ml,血浆中TNF-α和IL-6的水平分别为(207±29)pg/ml和(213±33)pg/ml,显著高于B组犤(521±76)pg/ml和(572±54)pg/ml,(113±18)pg/ml和(147±22)pg/ml犦及C组犤(72±13)pg/ml和(118±22)pg/ml,(67±10)pg/ml和(109±18)pg/ml犦(P均<0.05)。结论内毒素血症早期K释放的TNF-α和IL-6在回肠黏膜损害的启动和进程中可能起重要作用。
Objective To investigate the effect of inflammatory cytokines secreted by Kupffer cells (KC) on the lesion and the integrity of small intestinal mucosa after using phagocytic blockade gadolinium chloride (GdCl3) during early endotoxemia. Methods Thirty Wistar rats were divided into 3 groups.Group A: rats were injected only with endotoxin (E coli O111∶B4, a dose of 12 mg/kg);Group B:rats were treated intravenously with 25 mg/kg GdCl3 24 h prior to endotoxin injection; Group C: sham operation as control. In order to collect bile, bile duct cannulation was carried out in all the rats and bile was collected externally. Morphological change of ileum was observed under optical microscope. KC was isolated by collagenase perfusion and expression of TNF α and IL 6 mRNA in KC was determined with RT PCR. Levels of TNF α and IL 6 in bile and plasma were determined with ELISA kit. Results Neutrophil margination, superficial necrosis of the villi, sloughing of epithelium, and complete absence of some villi in ileum were observed in Group A. All these features of intestinal damage were improved obviously in Group B. The histological appearance was normal in Group C. Expression of TNF α and IL 6 mRNA decreased significantly in Group B as compared to Group A(P< 0.05).There was no expression of TNF α and IL 6 mRNA in Group C. In Group A, bile levels of TNF α and IL 6 were (1032±107)pg/ml and(1185±127)pg/ml;and plasma levels of TNF α and IL 6 were (207±29) pg/ml and(213±33)pg/ml respectively. In Group B, bile levels of TNF α and IL 6 were(521±76)pg/ml and(572±54)pg/ml; and plasma levels of TNF α and IL 6 were(113±18) pg/ml and(147±22)pg/ml respectively, which decreased significantly as compared to Group A (all P< 0.05). Low levels of TNF α and IL 6 were detected in bile [(72±13)pg/ml and(118±22) pg/ml] and plasma [(67±10)pg/ml and(109±18)pg/ml] in Group C.Conclusion KCs can release cytokines TNF α and IL 6 and play a crucial role in the initiation and progression of intestinal damage during early endotoxemia.
出处
《中华胃肠外科杂志》
CAS
2002年第2期149-152,共4页
Chinese Journal of Gastrointestinal Surgery
基金
国家自然科学基金资助项目(39970719
30170919)
