摘要
采用流式细胞术对经血小板特异性单克隆抗体和碘化丙啶(PI)双标染色的人正常骨髓巨核细胞DNA倍体性进行了分折。经Percoll(≤1.050g/ml)分离后,骨髓中血小板GPⅨ阳性巨核细胞明显升高,而在去除巨核细胞骨髓中(>1.050g/ml)血小板GPⅨ阳性巨核细胞的含量极少。未分离骨髓中和经Percoll分离骨髓中血小板GPⅨ阳性巨核细胞DNA倍体分布无明显差异,其DNA主次峰依次为16N(36％)和32N(24％),2N和4N巨核细胞占23％左右,有趣的是在经Percoll分离骨髓中2N和4N血小板GPⅨ阳性巨核细胞却低于血小板GPⅡb/Ⅲa和血小板GPⅢa阳性巨核细胞。
Using a double labelling technique with platelet specific monoclonal antibody for identification of megakaryocytes and vvith propidium iodide (PI) for megakaryocyte DNA staining, megakaryocyte DNA content and ploidy distribution in human nonnal bone marrovv were analysed by flow cytometry. Frequen-cy of megakaryocytes bearing platelet GPK in unfractionated bone marrow was low (0.1%). After separa-tion through Percoll, increased frequency (0.92%) of megakaryocytes expressing GPK was detected in the megakaryocyte-enriched low-density fraction (<1.050g/ml), which was 9 fold higher than that in unfractionated bone marrow. Meanwhile the celis expressing GPK decreased to an extremely low Ievel in megakaryocyte-depleted high-density bone marrow fraction (<1.050g/ml). There was no difference of platelet GPK positive megakaryocytes DNA ploidy distribution between in unfractionated bone marrow and in megakaryocyte-enriched low-density fraction. The most frequent DNA ploidy class was 16N (36%), the second one was 32N (24%), while 2N and 4N megakaryocytes amounted to about 23%. lnterestingly, the frequency of GPK positive megakaryocytes with 2N and 4N ploidy in the megakaryocyte-enriched low-density fraction was lower than that of platelet GPⅡb/ Ⅲa and GPⅢa positive megakaryocytes respectively.
出处
《中国实验血液学杂志》
CAS
CSCD
1996年第1期71-76,共6页
Journal of Experimental Hematology
