摘要
目的探讨宫内缺氧对胚胎大鼠大脑皮质神经元内c-Fos和一氧化氮合酶(nitric oxide syntbase,NOS)表达的影响。方法将8只孕期15天的孕鼠随机分为对照组和缺氧组各4只,缺氧组孕鼠采用低张性缺氧模型致鼠胚宫内缺氧1小时,两组鼠胚大脑组织经c-Fos和NOS免疫组化双标染色后,进行观察分析。结果缺氧组大脑皮质c-Fos阳性细胞、NOS阳性细胞和c-Fos/NOS双染阳性细胞均比对照组明显增多(P<0.05)。结论缺氧可刺激鼠胚大脑神经元内c-Fos和NOS的表达。
Objective To study the effect of hypoxia in uterus on the expression of c-Fos and nitric oxide synthase（NOS） in neurons of cerebral cortex from rats＇ embryos. Methods 8 conceiving （15d） female Wistar rats were divided randomly into 2 groups;the control group（CG） and the hypoxia group（HG）. The rats embryos of HG were in hypotonic hypoxia in uterus. The expression of c-Fos and NOS in cerebrum tissue of rats＇embryos was studied with immunohistochemistry double staining and the results were observed and analyzed under light microscope. Results The postive c- Fos cells, NOS cells and c-Fos/NSE cells in HG were more than that of CG（P〈0. 05） respectively. Conclusion Hypoxia could stimulate the expression of c-Fos and NOS in neurons of cerebral cortex from rats＇ embryos.
出处
《四川解剖学杂志》
2006年第1期27-29,共3页
Sichuan Journal of Anatomy
基金
四川省教育厅资助课题(川教计【2001】149-01LA40)
