摘要
介绍分离、纯化大鼠肝细胞 (用胶元酶二步灌流和Percoll不连续密度离心 )及分离后的培养 .此方法可靠易行 ,可制备出质量合格的肝细胞 (实质细胞 ) .讨论了保证分离和纯化成功的一些关键步骤、条件和原理 .提出分离肝细胞应首选胶元酶Ⅳ ,Percoll的渗透压和pH也是影响肝细胞的漂浮密度和存活能力的因素 .
Introduced were the isolation and purification methods of rat hepatocytes(two steps of collagenase perfusion and Percoll unconsecutive density centrifuge)and cultivation after isolation,which were simple and reliable to supply hepatocytes with good quality and quantity.To guarantee accurate results,some key steps,conditions and their mechanisms were discussed in detail.Collagenase Ⅳ was supposed to be the best collagenases for rat hepatocytes isolation and osmolality.pH of Percoll also had effect on float density and viability of hepatocytes.
出处
《昆明医学院学报》
2004年第2期114-116,共3页
Journal of Kunming Medical College
关键词
大鼠
肝细胞
分离
培养
胶元酶
Rat hepatocyte
Collagenase
Perfusion
Percoll
