摘要
目的研究DNA修复基因XPA rs1800975和XPD rs13181与宁夏汉族男性不育风险的相关性。方法采用病例-对照研究设计,应用PCR-RFLP技术分析353例男性不育患者和388例生育男性XPA基因rs1800975和XPD基因rs13181基因型,分析基因型频率、等位基因频率与男性不育的关系。结果 XPA基因rs1800975(A>G)AA、AG和GG三种基因型在病例组和对照组中的频率分别为25.8%、51.3%、22.9%和25.0%、53.4%、21.6%,rs1800975(A>G)基因型及等位基因频率在病例组和对照组中的分布均无统计学差异(P>0.05)。XPD基因rs13181(T>G)TT、TG和GG三种基因型在病例组和对照组中频率分别为83.9%、15.3%、0.8%和86.1%、13.9%、0.0%,rs13181(T>G)基因型和等位基因频率在病例组和对照组中的分布无统计学差异(P>0.05)。结论 XPA基因rs1800975和XPD基因rs13181可能不是宁夏汉族男性不育的遗传易感因素。
Objective To evaluate the relationship between DNA repair gene XPA rs1800975,XPD rs13181 and the risk of male infertility in Ningxia Han population. Methods PCR- restriction fragment length polymorphism( PCR- RFLP) method was used to geneotype XPA rs1800975 and XPD s13181 in 353 infertile patients and 388 fertile male control. Results The AA,AG and AA genotype frequencies of XPA rs1800975( A > G) in patients and control group were 25. 8%,51. 3%,22. 9% and 25. 0%,53. 4%,21. 6% respectively. There was no significant difference between two groups( P = 0. 985). The TT,TG and GG genotype frequencies of XPD rs13181( T > G) in patients and control group were 83. 9%,15. 3%,0. 8% and 86. 1%, 13. 9%,0. 0% respectively. There was no significant difference between two groups( P = 0. 161). Conclusion XPA rs1800975 and XPD rs13181 may not be the genetic risk factors of male infertility in Ningxia Han population.
出处
《宁夏医科大学学报》
2013年第10期1079-1082,1194,共5页
Journal of Ningxia Medical University
基金
宁夏生殖与遗传重点实验室开放课题(SY200809)
宁夏医科大学"博士学位建设学科"开放课题(KF-2010-22)
关键词
男性不育
基因多态性
单核苷酸
XPA
XPD
male infertility
gene polymorphism
single nucleotide polymorphism
XPA
XPD
