【目的】挖掘控制玉米体细胞再生的关键基因,为解析植物细胞命运决定的遗传机制,打破体细胞再生的基因型依赖性奠定基础。【方法】以再生能力强的玉米自交系JK11为植物材料,通过对其幼胚进行组织培养,鉴定胚性愈伤诱导率、绿化愈伤率和...【目的】挖掘控制玉米体细胞再生的关键基因,为解析植物细胞命运决定的遗传机制,打破体细胞再生的基因型依赖性奠定基础。【方法】以再生能力强的玉米自交系JK11为植物材料,通过对其幼胚进行组织培养,鉴定胚性愈伤诱导率、绿化愈伤率和绿苗再分化率3个体细胞再生相关性状。同时,利用RNA-seq技术对处于4个不同分化时期(0,5,10和15 d)的培养物进行测序,并分别开展比较转录组分析(JK0 vs JK5、JK0 vs JK10和JK0 vs JK15),筛选共同差异表达基因进行功能注释。【结果】自交系JK11的胚性愈伤诱导率、绿化愈伤率和绿苗再分化率的均值分别达到了97.70%,93.91%和86.79%,说明该材料可潜在用于遗传转化。转录组测序共获得12个文库的98.27 Gb高质量数据,平均每个文库的数据量达到了8.19 Gb。比较转录组分析筛选共同差异表达基因3789个,并划分为6个共表达模块。富集分析结果显示,这些基因主要参与到光合作用、类苯丙烷生物合成、淀粉和蔗糖代谢及植物激素信号转导等通路。此外,蛋白互作网络分析从中鉴定了1个核心节点Zm00001d021784及其得分最高的6个辐射节点,作为控制该性状的关键候选基因,qPCR验证结果显示预测的关键候选基因是可靠的。【结论】筛选体细胞再生中的共同差异表达基因3789个,其中包括Zm00001d021784在内的7个基因被鉴定为关键候选基因。展开更多
Embryonic stem cells possess fascinating capacity of self-renewal and developmental potential,leading to significant progress in understanding the molecular basis of pluripotency,disease modeling,and reprogramming tec...Embryonic stem cells possess fascinating capacity of self-renewal and developmental potential,leading to significant progress in understanding the molecular basis of pluripotency,disease modeling,and reprogramming technology.Recently,2-cell-like embryonic stem cells(ESCs)and expanded potential stem cells or extended pluripotent stem cells(EPSCs)generated from early-cleavage embryos display some features of totipotent embryos.These cell lines provide valuable in vitro models to study underlying principles of totipotency,cell plasticity,and lineage segregation.In this review,we summarize the current progress in this filed and highlight the application potentials of these cells in the future.展开更多
Pluripotent stem cells(PSCs)are useful for developmental and translational research because they have the potential to differentiate into all cell types of an adult individual.Pigs are one of the most important domest...Pluripotent stem cells(PSCs)are useful for developmental and translational research because they have the potential to differentiate into all cell types of an adult individual.Pigs are one of the most important domestic ungulates,commonly used for food and as bioreactors.Generating stable pluripotent porcine PSC lines remains challenging.So far,the pluripotency gene network of porcine PSCs is poorly understood.Here we found that TBX3-derived induced pluripotent stem cells(iPSCs)closely resemble porcine 4-cell embryos with the capacity of totipotent-like stem cells(TLSCs).Interestingly,our data suggest that TBX3 facilitates the activation of H3K4me3 methyltransferase,specifically MLL1.Subsequent investigations revealed that the porcine 4-cell specific gene,MCL1,is a key downstream effector of the TBX3-MLL1 axis.Together,our study of the TBX3 regulatory network is helpful in the understanding of the totipotency characteristics of pigs.展开更多
以源于二倍体梨品种Fertility(Pyrus communis L.)通过秋水仙碱离体诱变体细胞染色体加倍获得的不同同源多倍体无性系为试材,以离体叶片为外植体,观察研究了不同倍性无性系叶片的不定梢再生能力。结果表明,多倍体的不定梢再生率显著低...以源于二倍体梨品种Fertility(Pyrus communis L.)通过秋水仙碱离体诱变体细胞染色体加倍获得的不同同源多倍体无性系为试材,以离体叶片为外植体,观察研究了不同倍性无性系叶片的不定梢再生能力。结果表明,多倍体的不定梢再生率显著低于二倍体的再生率。不同多倍体无性系的不定梢再生能力也存在显著差异。三倍体无性系3x-3和四倍体无性系4x-4不能诱导产生不定梢。表明器官发生能力下降或植物细胞全能性的丧失与细胞染色体多倍体化有关。展开更多
Mouse embryonic stem cells(mESCs)are ideal tools for studying developmental biology and genetics because of their stemness characteristics;however,they cannot differentiate into extraembryonic lineages because of thei...Mouse embryonic stem cells(mESCs)are ideal tools for studying developmental biology and genetics because of their stemness characteristics;however,they cannot differentiate into extraembryonic lineages because of their origin.Many groups have attempted to derive extended pluripotent stem cells,which have the ability to differentiate into extraembryonic fates in addition to embryonic lineages[1].Recently,some totipotent-like stem cells with developmental potential for placental and yolk sac tissues have been reported[[2],[3],[4],[5]],strongly impacting developmental biology.The researchers focused on optimizing the medium by introducing some chemical compounds or inhibitors.However,the precise regulation of totipotency acquisition remains unknown and is a subject of great interest.Are there any key regulatory genes that govern totipotency activation?展开更多
文摘【目的】挖掘控制玉米体细胞再生的关键基因,为解析植物细胞命运决定的遗传机制,打破体细胞再生的基因型依赖性奠定基础。【方法】以再生能力强的玉米自交系JK11为植物材料,通过对其幼胚进行组织培养,鉴定胚性愈伤诱导率、绿化愈伤率和绿苗再分化率3个体细胞再生相关性状。同时,利用RNA-seq技术对处于4个不同分化时期(0,5,10和15 d)的培养物进行测序,并分别开展比较转录组分析(JK0 vs JK5、JK0 vs JK10和JK0 vs JK15),筛选共同差异表达基因进行功能注释。【结果】自交系JK11的胚性愈伤诱导率、绿化愈伤率和绿苗再分化率的均值分别达到了97.70%,93.91%和86.79%,说明该材料可潜在用于遗传转化。转录组测序共获得12个文库的98.27 Gb高质量数据,平均每个文库的数据量达到了8.19 Gb。比较转录组分析筛选共同差异表达基因3789个,并划分为6个共表达模块。富集分析结果显示,这些基因主要参与到光合作用、类苯丙烷生物合成、淀粉和蔗糖代谢及植物激素信号转导等通路。此外,蛋白互作网络分析从中鉴定了1个核心节点Zm00001d021784及其得分最高的6个辐射节点,作为控制该性状的关键候选基因,qPCR验证结果显示预测的关键候选基因是可靠的。【结论】筛选体细胞再生中的共同差异表达基因3789个,其中包括Zm00001d021784在内的7个基因被鉴定为关键候选基因。
基金supported by the National Natural Science Foundation of China(31970758)National Key R&D Program of China(2016YFA0102200,2017YFA0103301,2018YFC1004001)。
文摘Embryonic stem cells possess fascinating capacity of self-renewal and developmental potential,leading to significant progress in understanding the molecular basis of pluripotency,disease modeling,and reprogramming technology.Recently,2-cell-like embryonic stem cells(ESCs)and expanded potential stem cells or extended pluripotent stem cells(EPSCs)generated from early-cleavage embryos display some features of totipotent embryos.These cell lines provide valuable in vitro models to study underlying principles of totipotency,cell plasticity,and lineage segregation.In this review,we summarize the current progress in this filed and highlight the application potentials of these cells in the future.
基金funded by the National Basic Research Program of China(2022YFD1302200,2023YFF1000904,2023ZD0404303 and 2021YFD1200301)the National Natural Science Foundation of China(32072806,32372970 and 32002246)+2 种基金the Program of Shaanxi Province Science and Technology Innovation Team,China(2019TD-036)the Key Technologies Demonstration of Animal Husbandry in Shaanxi Province,China(20221086 and 20230978)the Inner Mongolia Autonomous Region Open Competition Project,China(2022JBGS0025).
文摘Pluripotent stem cells(PSCs)are useful for developmental and translational research because they have the potential to differentiate into all cell types of an adult individual.Pigs are one of the most important domestic ungulates,commonly used for food and as bioreactors.Generating stable pluripotent porcine PSC lines remains challenging.So far,the pluripotency gene network of porcine PSCs is poorly understood.Here we found that TBX3-derived induced pluripotent stem cells(iPSCs)closely resemble porcine 4-cell embryos with the capacity of totipotent-like stem cells(TLSCs).Interestingly,our data suggest that TBX3 facilitates the activation of H3K4me3 methyltransferase,specifically MLL1.Subsequent investigations revealed that the porcine 4-cell specific gene,MCL1,is a key downstream effector of the TBX3-MLL1 axis.Together,our study of the TBX3 regulatory network is helpful in the understanding of the totipotency characteristics of pigs.
文摘以源于二倍体梨品种Fertility(Pyrus communis L.)通过秋水仙碱离体诱变体细胞染色体加倍获得的不同同源多倍体无性系为试材,以离体叶片为外植体,观察研究了不同倍性无性系叶片的不定梢再生能力。结果表明,多倍体的不定梢再生率显著低于二倍体的再生率。不同多倍体无性系的不定梢再生能力也存在显著差异。三倍体无性系3x-3和四倍体无性系4x-4不能诱导产生不定梢。表明器官发生能力下降或植物细胞全能性的丧失与细胞染色体多倍体化有关。
基金supported by the National Key R&D Program of China,Synthetic Biology Research(2024YFA0917400)the National Natural Science Foundation of China(82371671,82201840,and 82301879)+4 种基金the Fundamental Research Funds for the Central Universities(63223030)the Institute of Transplantation Medicine(NKTM2023003)the Natural Science Foundation of Tianjin City(22JCZDJC00480)the Key Project of Tianjin Science and Technology(24ZXZSSS00150)the Shanghai Key Laboratory of Reproduction and Development(Redlab202401).We gratefully thank Ning Liu and Qian Liu of the instrument platform and the State Key Laboratory of Medicinal Chemical Biology of Nankai University for technical support.
文摘Mouse embryonic stem cells(mESCs)are ideal tools for studying developmental biology and genetics because of their stemness characteristics;however,they cannot differentiate into extraembryonic lineages because of their origin.Many groups have attempted to derive extended pluripotent stem cells,which have the ability to differentiate into extraembryonic fates in addition to embryonic lineages[1].Recently,some totipotent-like stem cells with developmental potential for placental and yolk sac tissues have been reported[[2],[3],[4],[5]],strongly impacting developmental biology.The researchers focused on optimizing the medium by introducing some chemical compounds or inhibitors.However,the precise regulation of totipotency acquisition remains unknown and is a subject of great interest.Are there any key regulatory genes that govern totipotency activation?
