摘要
选择山羊8细胞期到桑椹胚期的正常胚胎或重构胚的卵裂球,或选择胚泡期胚胎的内细胞团细胞作为供核,并以LRH注射后26—28h的去核成熟卵球作为受体,制备重构胚或再重构胚,琼脂糖包埋,植入山羊输卵管内,体内培养4—6天发育结果表明:不同发育时期(8细胞期至胚泡期)的正常胚胎细胞核或重构胚细胞核中,至少有部分细胞核均保留着发育的全能性,这些细胞核在母系基因表达产物的调控下,实现重新编程,启动并完成正常发育的全过程。
Goat reconstituted embryos (REs) have been produced by electrofusion-me-diated nuclear transplantation method. Single cell derived from normal embryos or REs developed to 8-cell morula stage or the inner cell mass (ICM) of early blastocyst stage was used to fuse with enucleated mature egg (26-28 hrs after injection of LRH). According to the results summarized in table 1 and 2, we decided to adopt the method to embed REs in agarose and then transfer into goat oviduct lumen of host mother for 4-6 days in vivo culture. Normal
fertilized eggs seem to develop synchronously, but that of REs are not. Tables 4 and 5 reveal that REs and embryos reconstituted successively can develop normally, no significant difference was found among their development rates. All these experimental results indicate that nuclei of some blastomeres from normal embryos or REs (derived from eight cells to morula or ICM) retained their totipotency for further development. These nuclei can be reprogrammed in host ooplasm, and developed to term.
出处
《实验生物学报》
CSCD
1995年第2期147-155,共9页
Acta Biologiae Experimentalis Sinica
基金
国家863高技术研究发展计划(生物技术领域)资助项目
关键词
山羊
重构胚
早期发育
发育
Goat reconstituted embryo. Early development. Totipotency
