Understanding the based-on drug or drug conjugates to reach the beneficial optimally recognized by the immune system requires multidisciplinary approaches and detailed of albumin as the key circulating a transporting/...Understanding the based-on drug or drug conjugates to reach the beneficial optimally recognized by the immune system requires multidisciplinary approaches and detailed of albumin as the key circulating a transporting/transmission and antioxidant protein of blood drug interaction. Albumin, in reduced form (mercaptoalbumin, HMA), with antioxidant ability and alterations/deteriorations in the redox status of human serum albumin (HSA) under oxidative stress formation in infection diseases and its complications strongly modifies albumin antioxidant capacity. The aim of this study was the investigation of carbonyl/oxidative stress and pseudo-esterase activity of mercaptolbumin and oxidized HSA models. HSA (P. pastoris) purchased from MedChemExpress (USA) was used for study to model oxidative stress, HSA in reduced (intact) form was treated with H2O2, tert-butylhydroperoxide (t-BHP) and chloramine T (CT). The content of HSA-bound carbonyl groups decreased in under treatment with t-BPH- and CT-reduced HSA and more less extent in case of H2O2-treated. Fatty acid-free HSA and mercaptoalbumin (HMA) advanced oxidation protein products (AOPP) concentrations were significantly lower than in H2O2 loading reduced HSA by 123% and 235%, respectively. The total thiols level was lower in HMA + CT compared to reduced HMA by 51% and even increased after treatment of HMA with H2O2. Pseudo-esterase activity of HMA maintains >65% in the presence of hydroperoxide and occurs pronounced loss in the presence of chloramine T. Hydrogen peroxide at physiological concentration about 10 μM occurs less damage of reduced from of HSA then t-BPH and CT, and unlike t-BPH and CT, without significantly changes in pseudo-esterase activities.展开更多
Metal-organic frameworks(MOFs)attract broad interests in mercury(Hg)ion adsorption field,while unreasonable distribution of active groups commonly restricts their utilization efficiency.In this work,we constructed a n...Metal-organic frameworks(MOFs)attract broad interests in mercury(Hg)ion adsorption field,while unreasonable distribution of active groups commonly restricts their utilization efficiency.In this work,we constructed a new MOF(TYUST-6)with dense thiol-rich traps in the 1D pore wall.This accessible channel and rational distribution of thiols allow the smooth diffusion of Hg ions and thereby result in a high Langmuir adsorption capacity of 1347.6 mg/g,almost reaching the theoretical maximum(1444.3 mg/g).Adsorption equilibrium needs 10 and 30 min at the initial concentrations of 10 and 100 mg/L,respectively.Common co-existing ions and solution pH show almost negligible interferences on the adsorption,and adsorbent regeneration can be well achieved.Combining experimental characterizations and theoretical calculations,the thiol groups in the pore wall are proved to be the dominant interaction sites.Thus,this work reports a novel high-capacity adsorbent for Hg^(2+),and proposes a feasible guideline for designing effective adsorbents.展开更多
文摘Understanding the based-on drug or drug conjugates to reach the beneficial optimally recognized by the immune system requires multidisciplinary approaches and detailed of albumin as the key circulating a transporting/transmission and antioxidant protein of blood drug interaction. Albumin, in reduced form (mercaptoalbumin, HMA), with antioxidant ability and alterations/deteriorations in the redox status of human serum albumin (HSA) under oxidative stress formation in infection diseases and its complications strongly modifies albumin antioxidant capacity. The aim of this study was the investigation of carbonyl/oxidative stress and pseudo-esterase activity of mercaptolbumin and oxidized HSA models. HSA (P. pastoris) purchased from MedChemExpress (USA) was used for study to model oxidative stress, HSA in reduced (intact) form was treated with H2O2, tert-butylhydroperoxide (t-BHP) and chloramine T (CT). The content of HSA-bound carbonyl groups decreased in under treatment with t-BPH- and CT-reduced HSA and more less extent in case of H2O2-treated. Fatty acid-free HSA and mercaptoalbumin (HMA) advanced oxidation protein products (AOPP) concentrations were significantly lower than in H2O2 loading reduced HSA by 123% and 235%, respectively. The total thiols level was lower in HMA + CT compared to reduced HMA by 51% and even increased after treatment of HMA with H2O2. Pseudo-esterase activity of HMA maintains >65% in the presence of hydroperoxide and occurs pronounced loss in the presence of chloramine T. Hydrogen peroxide at physiological concentration about 10 μM occurs less damage of reduced from of HSA then t-BPH and CT, and unlike t-BPH and CT, without significantly changes in pseudo-esterase activities.
基金supported by the National Natural Science Foundation of China(No.22208230)Fundamental Research Program of Shanxi Province(No.202103021223281)。
文摘Metal-organic frameworks(MOFs)attract broad interests in mercury(Hg)ion adsorption field,while unreasonable distribution of active groups commonly restricts their utilization efficiency.In this work,we constructed a new MOF(TYUST-6)with dense thiol-rich traps in the 1D pore wall.This accessible channel and rational distribution of thiols allow the smooth diffusion of Hg ions and thereby result in a high Langmuir adsorption capacity of 1347.6 mg/g,almost reaching the theoretical maximum(1444.3 mg/g).Adsorption equilibrium needs 10 and 30 min at the initial concentrations of 10 and 100 mg/L,respectively.Common co-existing ions and solution pH show almost negligible interferences on the adsorption,and adsorbent regeneration can be well achieved.Combining experimental characterizations and theoretical calculations,the thiol groups in the pore wall are proved to be the dominant interaction sites.Thus,this work reports a novel high-capacity adsorbent for Hg^(2+),and proposes a feasible guideline for designing effective adsorbents.
