摘要
角蛋白基水凝胶在生物医用材料和化妆品领域得到越来越多的应用,而不同工艺技术提取的角蛋白原料之间的相对分子质量及其分布、活性巯基基团含量等存在明显差异,如何选择角蛋白原料制备角蛋白水凝胶值得研究。文章以硫化钠法、L-半胱氨酸法、亚硫酸氢钠法和过氧乙酸法所提取羊毛角蛋白为原料,分别制备了四种角蛋白基水凝胶,并依次命名为KH1、KH2、KH3和KH4。根据不同提取工艺所得角蛋白结构和性能参数,研究角蛋白基水凝胶的孔隙结构、压缩机械性能、溶胀性能、流变行为、黏度、pH及H_(2)O_(2)响应性等性能。实验结果显示,KH1、KH2的孔径分布在30~90μm,而KH3和KH4的孔径分布则在100~200μm;KH1和KH2展现出较高的储能模量和良好的抵抗压缩性能,而KH4的储能模量最低,容易被压缩。此外,四种角蛋白水凝胶均展现出良好的pH和H_(2)O_(2)响应性。对如何选择角蛋白原料制备角蛋白基生物材料具有一定的指导意义。
Keratin-based hydrogels have been used more and more in the fields of biomedical materials and cosmetics.However,there are obvious differences in the molecular weight and its distribution as well as the content of active sulfhydryl groups of keratin raw materials extracted by different technologies.In this paper,four kinds of keratin-based hydrogels were prepared from wool keratin extracted by sodium sulfide method,L-cysteine method,sodium bisulfite method and peracetic acid method,respectively,and named KH1,KH2,KH3 and KH4 in sequence.The pore structure,compressive mechanical properties,swelling properties,rheological behavior,viscosity,pH and H_(2)O_(2) responsiveness of keratin-based hydrogels were studied according to the structure and properties of keratin obtained by different extraction processes.The experimental results show that the pore sizes of KH1 and KH2 range from 30μm to 90μm,while those of KH3 and KH4 range from 100μm to 200μm.KH1 and KH2 show high energy storage moduli and good resistance to compression,while KH4 has the lowest energy storage modulus and is easily compressed.In addition,all four keratin hydrogels showed good pH and H_(2)O_(2) responsiveness.This study has certain guiding significance for how to select keratin raw materials to prepare keratin-based biomaterials.
作者
黎鑫
邹蝶
程海明
LI Xin;ZOU Die;CHENG Haiming(National Engineering Laboratory for Clean Technology of Leather Manufacture,Sichuan University,Chengdu 610065,China;Key Laboratory of Leather Chemistry and Engineering of Ministry of Education,Sichuan University,Chengdu 610065,China)
出处
《皮革科学与工程》
北大核心
2025年第5期15-21,共7页
Leather Science and Engineering
基金
国家自然科学基金面上项目(22178231)。
关键词
角蛋白
水凝胶
孔隙结构
力学性能
巯基
角蛋白基生物材料
keratin
hydrogel
pore structure
mechanical property
thiol group
keratin-based biomaterials
