Pumpkin was used as vegetables.They are used popularly in the meals or making drinks because pumpkin contains vitamins,fiber,mineral,protein,glucose and lipid.Besides,pumpkin is not only nourishing,but also is preciou...Pumpkin was used as vegetables.They are used popularly in the meals or making drinks because pumpkin contains vitamins,fiber,mineral,protein,glucose and lipid.Besides,pumpkin is not only nourishing,but also is precious drugs to cure diseases.In this article,we analyzed some ingredients including heavy metals and NO3-in soil,water and pumpkin samples from households in Loc Thanh Village,Bao Lam District,Lam Dong Province(pumpkin of households grown by using chemical fertilizers and pesticides).These samples showed that some heavy metals such as Cu(Copper),Pb(Plumb),Cd(Cadmium)and As(Arsen)exceeded the allowable limits.However,the pumpkin samples that we grew by using organic fertilizers showed a significant reduction in heavy metal content,within the safety limit according to Vietnamese standards.Thus,the using organic farming methods has resulted in better quality products than using chemical fertilizers and pesticides.展开更多
Pumpkin is of great economic importance not only as food resources but also as the most widely used rootstock to graft cucurbit crops.Pumpkin rootstock improves salt tolerance of cucumber scion through respiratory bur...Pumpkin is of great economic importance not only as food resources but also as the most widely used rootstock to graft cucurbit crops.Pumpkin rootstock improves salt tolerance of cucumber scion through respiratory burst oxidase homolog protein D(CmRbohD)-mediated reactive oxygen species(ROS)burst,which further enhances Na^(+)export and K^(+)uptake.Rboh D activation requires calcium signaling.However,the underlying mechanism remains largely unknown.In this study,we discovered two Rboh D members from pumpkin involved in the ROS burst at the early stage of salt stress.CmRbohD1 and CmRbohD2 were functionally redundant and double mutation significantly impaired salt tolerance in pumpkin.Overexpression of CmRbohD1 and CmRbohD2 mitigated salinity-induced damage and maintained a relatively low Na+content and high K+content.We screened the potential calcineurin B-like interacting protein kinases(Cm CIPKs)which could bind with CmRbohD1 and CmRbohD2.Our results revealed that two Rboh Ds formed complexes specifically with CmCIPK1,thereby bursting ROS production.Overexpression of CmCIPK1 promoted the early ROS burst under salt stress condition and maintained a relatively balanced Na+/K+homeostasis.Altogether,we proposed a CmCIPK1-CmRbohD1/D2 complex for pumpkin salt stress signaling transduction,which regulates the ROS burst and Na+/K+homeostasis.Our findings offer unprecedented insights into the general mechanism of pumpkin salt tolerance.展开更多
A cluster analysis was carried out based on Euclidean genetic distances through UPGMA method in Chinese pumpkin inbred lines.7 important agronomic traits of 46 Chinese pumpkin inbred lines were investigated.The result...A cluster analysis was carried out based on Euclidean genetic distances through UPGMA method in Chinese pumpkin inbred lines.7 important agronomic traits of 46 Chinese pumpkin inbred lines were investigated.The result indicated that 46 pumpkin inbred lines were clustered into 4 groups and the inter-groups distances was larger than that in intra-group.The genetic distances of parents were related to F1 performance and the results of cluster would increase effectiveness in the Chinese pumpkin crossing breeding.展开更多
[Objective] The research aimed to discuss the relationship between Glycine betaine and the salt-tolerance mechanism of different pumpkins and confirm the mechanism of betaine in salt-tolerance physiology.[Method] Taki...[Objective] The research aimed to discuss the relationship between Glycine betaine and the salt-tolerance mechanism of different pumpkins and confirm the mechanism of betaine in salt-tolerance physiology.[Method] Taking the seedlings of C.ficifolia and Qingli pumpkin as test materials,the effects of glycine betaine on the cell membrane permeability,MDA and root activity of two varieties of pumpkin seedlings under 300 mmol/L NaCl stress were studied.[Result] At suitable concentrations,glycine betaine could d...展开更多
[Objective] This paper aims to study a new method of extracting pumpkin polysaccharide from pumpkin. Single factor experiments were conducted to examine the effects of extracting time,temperature,the solid-liquid rati...[Objective] This paper aims to study a new method of extracting pumpkin polysaccharide from pumpkin. Single factor experiments were conducted to examine the effects of extracting time,temperature,the solid-liquid ratio and pH value on the extraction yield of polysaccharide from pumpkin. [Method] The best enzyme ratio and extraction conditions for complex enzymes extraction were determined through orthogonal tests. Scavenging ·OH and O-2 activities of pumpkin polysaccharides were also investigated by salicylic acid and improved self-oxidation of o-pheno methods respectively. [Results] The results showed that the biggest extraction yield of polysaccharide from pumpkin can be got when adding 1% cellulose enzyme,1.5% pectinase,1.0% papain and Na2HPO4-citric acid buffer solution (pH was 4.6),and oscillating for 30 min under water at 40 ℃ with the solid-liquid ratio of 1:30. In addition,pumpkin polysaccharides had a strong activity of eliminating ·OH,but very weak activity to scavenge O-2. [Conclusion] This study provided basic data for research and application of Pumpkin polysaccharide.展开更多
In this study,an orthogonal experiment was performed to explore the optimal extraction technique of water-soluble polysaccharides from pumpkin.Moreover,the polysaccharides＇ capabilities to scavenge ·OH and DPPH&...In this study,an orthogonal experiment was performed to explore the optimal extraction technique of water-soluble polysaccharides from pumpkin.Moreover,the polysaccharides＇ capabilities to scavenge ·OH and DPPH· and their gross reducing power was measured with Vc and BHT as references.In the orthogonal experiment,various factors including temperature,extraction time,solid-liquid ratio,were optimized.The results showed that the polysaccharides extracted from pumpkin are endowed with strong in vitro antioxidant properties.Our study provided references for the extraction of pumpkin polysaccharides and for their use as antioxidants.展开更多
[Objective] Pollen mother cell miosis and male gametophyte development of pumpkin were observed in this study, to provide some cytological basis for pumpkin anther or microspore culture. [Method] Ehrlich＇s hematoxyli...[Objective] Pollen mother cell miosis and male gametophyte development of pumpkin were observed in this study, to provide some cytological basis for pumpkin anther or microspore culture. [Method] Ehrlich＇s hematoxylin staining-methyl salicylate clearing technique was used for observation and research of the variation of cell structure and chromosomal behavior during pollen mother cell miosis and male gametophyte development of ‘Tianhong＇ pumpkin. [Result] The meiosis in pollen moth- er cells of pumpkin was simultaneous cytokinesis. In the process of nuclear division, nuclear membrane and nucleolus of pumpkin pollen mother cells gradually disappeared in the metaphase I and reappeared in telophase I , phragmoplast formed between the two generated crescent-shaped nuclei without cell wall, the phragmoplast gradually disappeared in the metaphase II and reappeared in telophase II. Phragmoplast spread outward from the center of spindle during the second division was connected with that formed on the central interface of two nuclei during the first division, cell wall of microspores generated from periphery to center. Most of the tetrads contained four sub-cells while a few contained extra small cells. During the period of uniuclete microspore at periphery, the single nucleolus split into 2-3 or more small nucleoli, mature pollen grain was two-celled. Mononucleate pollen cells were mostly appeared in the flower buds with length of 1.0-2.0 cm, which could be used as an important indicator to collect materials for anther or microspore culture. [Conclusion] This study laid the foundation for research of the cytogenetics of pumpkin.展开更多
[Objective] This study aimed to develop pumpkin sausage. [Method] The single-factor design and orthogonal design were adopted. In the traditional sausage,fresh pumpkin was added. Based on the results of sensory test a...[Objective] This study aimed to develop pumpkin sausage. [Method] The single-factor design and orthogonal design were adopted. In the traditional sausage,fresh pumpkin was added. Based on the results of sensory test and physical and chemical indexes determination, the optimum recipe for pumpkin sausage was determined. [Result] The optimum recipe for pumpkin sausage was as follows： 100% of pork（fat to lean ratio of 2∶8）, 45% of pumpkin particles, 6% of soy protein, 7% of sugar, 3.5% of salt, 1% of spice and 0.5% of monascus red pigment. [Conclusion]The successful preparation of pumpkin sausage will provide consumers with nutrition, health and snack meat product with comprehensive nutrition and unique flavor.展开更多
The No.601 watermelon (citrullus lanatus) seeds were treated with 25 keV N+ implantation at the dosage of 7.8 ×1016 ions/cm2. After treatment, watermelon seeds were incubated with 380μg/μl pumpkin (Cucubita, ma...The No.601 watermelon (citrullus lanatus) seeds were treated with 25 keV N+ implantation at the dosage of 7.8 ×1016 ions/cm2. After treatment, watermelon seeds were incubated with 380μg/μl pumpkin (Cucubita, maxima Duch) DNA solution at 35 ℃ for 5 hours. By two-generations of selection and resistance screening at seedling stage, one transformed material was selected out, whose rind color is similar to that of the donor pumpkin and whose size of seeds is between that of the donor and the receptor. Using AFLP (amplified fragment length polymorphism) technique, two polymorphic DNA fragments were amplified. This primarily testified that the donor DNA fragments/gene were introduced into the receptor cell and integrated into the genomic DNA of the receptor.展开更多
Process parameters of microwave assisted extraction （MAE） of the polysaccharides from pumpkin viz. extraction temperature, time and liquid-solid ratio were studied by using single factor and response surface methodo...Process parameters of microwave assisted extraction （MAE） of the polysaccharides from pumpkin viz. extraction temperature, time and liquid-solid ratio were studied by using single factor and response surface methodology method. The results showed that the liquid-solid ratio was the most important factor in polysaccharides yield, followed the extraction temperature was the least important factor. The optimum microwave assisted extraction co by ndi extraction time, and tions for the highest polysaccharides yield from pumpkin （16.76%-4-0.38%） were obtained by using the response surface methodology with extraction time of 29 min, an extraction temperature of 79 ℃ and a liquid-solid ratio of 22 mL·g^-1. Validation experiment result well agreed with predicted value.展开更多
The polysaccharides from pumpkin fruit （PP） were obtained and purified by hot-water extraction, anion-exchange chromatography, and gel column chromatography. The physicochemical properties of PP were determined by g...The polysaccharides from pumpkin fruit （PP） were obtained and purified by hot-water extraction, anion-exchange chromatography, and gel column chromatography. The physicochemical properties of PP were determined by gel filtration chromatography, gas chromatography, fourier transform infrared （FTIR） spectroscopy, and nuclear magnetic resonance （NMR） spectroscopy. Results indicated that the molecular weight of PP was about 23 kDa and PP was composed of D-Arabinose, D-Marmose, D-Glucose, and D-Galactose with a molar ratio of 1 ： 7.79 ： 70.32 ： 7.05. FTIR and NMR spectra indicated that PP was the polysaccharide containing pyranose ring. Additionally, PP protected islets cells from streptozotocin （STZ） injury in vitro via increasing the levels of super-oxide dismutase （SOD） and malondialdehyde （MDA） and reducing the production of NO. The experiment of reverse transcriptase-polymerase chain reaction further proved that PP inhibited apoptosis via modulating the expression of Bax/Bcl-2 in STZ-damaged islet cells. In conclusion, PP could be explored as a novel agent for the treatment of diabetes mellitus.展开更多
文摘Pumpkin was used as vegetables.They are used popularly in the meals or making drinks because pumpkin contains vitamins,fiber,mineral,protein,glucose and lipid.Besides,pumpkin is not only nourishing,but also is precious drugs to cure diseases.In this article,we analyzed some ingredients including heavy metals and NO3-in soil,water and pumpkin samples from households in Loc Thanh Village,Bao Lam District,Lam Dong Province(pumpkin of households grown by using chemical fertilizers and pesticides).These samples showed that some heavy metals such as Cu(Copper),Pb(Plumb),Cd(Cadmium)and As(Arsen)exceeded the allowable limits.However,the pumpkin samples that we grew by using organic fertilizers showed a significant reduction in heavy metal content,within the safety limit according to Vietnamese standards.Thus,the using organic farming methods has resulted in better quality products than using chemical fertilizers and pesticides.
基金supported by National Natural Science Foundation of China(Grant Nos.32072653,32372794,31772357)Natural Science Foundation of Hubei Province(Grant No.2019CFA017)+1 种基金Ningbo Scientific and Technological Project(Grant No.2021Z006)the Fundamental Research Funds for the Central Universities(Grant No.2662023YLPY008)。
文摘Pumpkin is of great economic importance not only as food resources but also as the most widely used rootstock to graft cucurbit crops.Pumpkin rootstock improves salt tolerance of cucumber scion through respiratory burst oxidase homolog protein D(CmRbohD)-mediated reactive oxygen species(ROS)burst,which further enhances Na^(+)export and K^(+)uptake.Rboh D activation requires calcium signaling.However,the underlying mechanism remains largely unknown.In this study,we discovered two Rboh D members from pumpkin involved in the ROS burst at the early stage of salt stress.CmRbohD1 and CmRbohD2 were functionally redundant and double mutation significantly impaired salt tolerance in pumpkin.Overexpression of CmRbohD1 and CmRbohD2 mitigated salinity-induced damage and maintained a relatively low Na+content and high K+content.We screened the potential calcineurin B-like interacting protein kinases(Cm CIPKs)which could bind with CmRbohD1 and CmRbohD2.Our results revealed that two Rboh Ds formed complexes specifically with CmCIPK1,thereby bursting ROS production.Overexpression of CmCIPK1 promoted the early ROS burst under salt stress condition and maintained a relatively balanced Na+/K+homeostasis.Altogether,we proposed a CmCIPK1-CmRbohD1/D2 complex for pumpkin salt stress signaling transduction,which regulates the ROS burst and Na+/K+homeostasis.Our findings offer unprecedented insights into the general mechanism of pumpkin salt tolerance.
文摘A cluster analysis was carried out based on Euclidean genetic distances through UPGMA method in Chinese pumpkin inbred lines.7 important agronomic traits of 46 Chinese pumpkin inbred lines were investigated.The result indicated that 46 pumpkin inbred lines were clustered into 4 groups and the inter-groups distances was larger than that in intra-group.The genetic distances of parents were related to F1 performance and the results of cluster would increase effectiveness in the Chinese pumpkin crossing breeding.
基金Supported by the National Natural Science Foundation of China(30660015)Key Laboratory of Vegetable Genetics and Physiology of Ministry of Agriculture of the People s Republic of China(2003-2005)~~
文摘[Objective] The research aimed to discuss the relationship between Glycine betaine and the salt-tolerance mechanism of different pumpkins and confirm the mechanism of betaine in salt-tolerance physiology.[Method] Taking the seedlings of C.ficifolia and Qingli pumpkin as test materials,the effects of glycine betaine on the cell membrane permeability,MDA and root activity of two varieties of pumpkin seedlings under 300 mmol/L NaCl stress were studied.[Result] At suitable concentrations,glycine betaine could d...
基金Supported by the Key Scientific and Technological Project of Henan Province (102102110157)the Scientific Research Found Project of Henan University of Urban Construction (2010JZD008)~~
文摘[Objective] This paper aims to study a new method of extracting pumpkin polysaccharide from pumpkin. Single factor experiments were conducted to examine the effects of extracting time,temperature,the solid-liquid ratio and pH value on the extraction yield of polysaccharide from pumpkin. [Method] The best enzyme ratio and extraction conditions for complex enzymes extraction were determined through orthogonal tests. Scavenging ·OH and O-2 activities of pumpkin polysaccharides were also investigated by salicylic acid and improved self-oxidation of o-pheno methods respectively. [Results] The results showed that the biggest extraction yield of polysaccharide from pumpkin can be got when adding 1% cellulose enzyme,1.5% pectinase,1.0% papain and Na2HPO4-citric acid buffer solution (pH was 4.6),and oscillating for 30 min under water at 40 ℃ with the solid-liquid ratio of 1:30. In addition,pumpkin polysaccharides had a strong activity of eliminating ·OH,but very weak activity to scavenge O-2. [Conclusion] This study provided basic data for research and application of Pumpkin polysaccharide.
基金Supported by the Key Science and Technology Project of Henan Province(102102110157)the Science Fund of Henan University of Urban Construction(2010JZD008)~~
文摘In this study,an orthogonal experiment was performed to explore the optimal extraction technique of water-soluble polysaccharides from pumpkin.Moreover,the polysaccharides＇ capabilities to scavenge ·OH and DPPH· and their gross reducing power was measured with Vc and BHT as references.In the orthogonal experiment,various factors including temperature,extraction time,solid-liquid ratio,were optimized.The results showed that the polysaccharides extracted from pumpkin are endowed with strong in vitro antioxidant properties.Our study provided references for the extraction of pumpkin polysaccharides and for their use as antioxidants.
基金Supported by Project of Jiangsu Provincial Department of Education (JHZD06-7)Qing Lan Project of Colleges and Universities in Jiangsu Province (2008 No.30)~~
文摘[Objective] Pollen mother cell miosis and male gametophyte development of pumpkin were observed in this study, to provide some cytological basis for pumpkin anther or microspore culture. [Method] Ehrlich＇s hematoxylin staining-methyl salicylate clearing technique was used for observation and research of the variation of cell structure and chromosomal behavior during pollen mother cell miosis and male gametophyte development of ‘Tianhong＇ pumpkin. [Result] The meiosis in pollen moth- er cells of pumpkin was simultaneous cytokinesis. In the process of nuclear division, nuclear membrane and nucleolus of pumpkin pollen mother cells gradually disappeared in the metaphase I and reappeared in telophase I , phragmoplast formed between the two generated crescent-shaped nuclei without cell wall, the phragmoplast gradually disappeared in the metaphase II and reappeared in telophase II. Phragmoplast spread outward from the center of spindle during the second division was connected with that formed on the central interface of two nuclei during the first division, cell wall of microspores generated from periphery to center. Most of the tetrads contained four sub-cells while a few contained extra small cells. During the period of uniuclete microspore at periphery, the single nucleolus split into 2-3 or more small nucleoli, mature pollen grain was two-celled. Mononucleate pollen cells were mostly appeared in the flower buds with length of 1.0-2.0 cm, which could be used as an important indicator to collect materials for anther or microspore culture. [Conclusion] This study laid the foundation for research of the cytogenetics of pumpkin.
文摘[Objective] This study aimed to develop pumpkin sausage. [Method] The single-factor design and orthogonal design were adopted. In the traditional sausage,fresh pumpkin was added. Based on the results of sensory test and physical and chemical indexes determination, the optimum recipe for pumpkin sausage was determined. [Result] The optimum recipe for pumpkin sausage was as follows： 100% of pork（fat to lean ratio of 2∶8）, 45% of pumpkin particles, 6% of soy protein, 7% of sugar, 3.5% of salt, 1% of spice and 0.5% of monascus red pigment. [Conclusion]The successful preparation of pumpkin sausage will provide consumers with nutrition, health and snack meat product with comprehensive nutrition and unique flavor.
基金This work was supported by National Key Project (96-538-01-01).
文摘The No.601 watermelon (citrullus lanatus) seeds were treated with 25 keV N+ implantation at the dosage of 7.8 ×1016 ions/cm2. After treatment, watermelon seeds were incubated with 380μg/μl pumpkin (Cucubita, maxima Duch) DNA solution at 35 ℃ for 5 hours. By two-generations of selection and resistance screening at seedling stage, one transformed material was selected out, whose rind color is similar to that of the donor pumpkin and whose size of seeds is between that of the donor and the receptor. Using AFLP (amplified fragment length polymorphism) technique, two polymorphic DNA fragments were amplified. This primarily testified that the donor DNA fragments/gene were introduced into the receptor cell and integrated into the genomic DNA of the receptor.
基金Supported by National Natural Science Foundation (31071579)Key Program of Heilongjiang Province Science Foundation (ZP201013)
文摘Process parameters of microwave assisted extraction （MAE） of the polysaccharides from pumpkin viz. extraction temperature, time and liquid-solid ratio were studied by using single factor and response surface methodology method. The results showed that the liquid-solid ratio was the most important factor in polysaccharides yield, followed the extraction temperature was the least important factor. The optimum microwave assisted extraction co by ndi extraction time, and tions for the highest polysaccharides yield from pumpkin （16.76%-4-0.38%） were obtained by using the response surface methodology with extraction time of 29 min, an extraction temperature of 79 ℃ and a liquid-solid ratio of 22 mL·g^-1. Validation experiment result well agreed with predicted value.
基金supported by the Natural Science Foundation Committee of China(NSFC 81202467)the Natural Science Foundation of Jiangsu Province in China(BK2012232)+2 种基金the Natural Science Research Project of Universities in Jiangsu Province of China(11KJB350004,06KJD310150,12KJB310012)the Natural Science Foundation of Nantong City of China(BK2011044)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘The polysaccharides from pumpkin fruit （PP） were obtained and purified by hot-water extraction, anion-exchange chromatography, and gel column chromatography. The physicochemical properties of PP were determined by gel filtration chromatography, gas chromatography, fourier transform infrared （FTIR） spectroscopy, and nuclear magnetic resonance （NMR） spectroscopy. Results indicated that the molecular weight of PP was about 23 kDa and PP was composed of D-Arabinose, D-Marmose, D-Glucose, and D-Galactose with a molar ratio of 1 ： 7.79 ： 70.32 ： 7.05. FTIR and NMR spectra indicated that PP was the polysaccharide containing pyranose ring. Additionally, PP protected islets cells from streptozotocin （STZ） injury in vitro via increasing the levels of super-oxide dismutase （SOD） and malondialdehyde （MDA） and reducing the production of NO. The experiment of reverse transcriptase-polymerase chain reaction further proved that PP inhibited apoptosis via modulating the expression of Bax/Bcl-2 in STZ-damaged islet cells. In conclusion, PP could be explored as a novel agent for the treatment of diabetes mellitus.
